Seed-preferred regulatory elements
AssigneePioneer Hi-Bred International, Inc.
InventorShane E. Abbitt
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Abstract
The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a tissue preferred promoter isolated from the sorghum legumin coding region. The sequences drive expression preferentially to seed tissue, and most preferably to endosperm tissue of a plant. A method for expressing a nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.
Claims12
An isolated regulatory element that drives transcription in a seed endosperm-preferred manner, wherein the regulatory element comprises
- the nucleotide sequence of SEQ ID NO:
- 1.
A method for expressing a nucleotide sequence in a plant cell, the method comprising:
- a) transforming a plant cell with an expression cassette, the expression cassette comprising an isolated regulatory element operably linked to a nucleotide sequence wherein the regulatory element comprises the nucleotide sequence as set forth in SEQ ID NO:
- 1;
- and
- b) growing the plant cell to express the nucleotide sequence.
Dependencies are inferred from the claim text.