Dehydrin gene from Avicennia marina responsible for conferring salt tolerance in plants
AssigneeM.S. Swaminathan Research Foundation
InventorsAjay Parida, Preeti Mehta Angela, Gayatri Venkatraman
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Abstract
The present invention relates to a method of producing salt-stress tolerant plants by transforming the plants with an isolated nucleic acid sequences encoding a dehydrin (DHN) protein. The invention further provides a transgenic plant expressing the dehydrin gene of Avicennia marina. Using functional genomics, this gene was derived from large-scale EST sequencing of the cDNA library of the salt tolerant mangrove Avicennia marina.
Claims14
An isolated polynucleotide comprising a polynucleotide sequence having at least 80% similarity to SEQ ID NO:
- 1 and coding for a dehydrin polypeptide having the amino acid sequence as shown in SEQ ID NO:
- 3.
An isolated polynucleotide comprising the polynucleotide sequence as set forth in SEQ ID NO:
- 1 and coding for a dehydrin polypeptide having the amino acid sequence as shown in SEQ ID NO:
- 3.
A recombinant construct for transforming plants to confer salt stress tolerance comprising a promoter selected from the group consisting of 35S CaMV, nopaline synthase, OCS, AdhI, AdhII and Ubi-1, wherein the promoter is operably linked to a polynucleotide comprising a polynucleotide sequence having at least 80% similarity to SEQ ID NO:
- 1 and coding for a dehydrin polypeptide having the amino acid sequence as shown in SEQ ID NO:
- 3.
A method of transformation of plants to confer salt stress tolerance, said method comprising transforming plants with a recombinant vector comprising a promoter operably linked to a polynucleotide comprising a polynucleotide sequence having at least 80% similarity to SEQ ID NO:
- 1 and coding for a dehydrin polypeptide having the amino acid sequence as shown in SEQ ID NO:
- 3, and wherein expression of the polynucleotide confers tolerance to salt stress in said plants.
A method of transforming plants, wherein the method comprises:
- (a) constructing a recombinant construct comprising a promoter selected from the group consisting of 35S CaMV, nopaline synthase, OCS, AdhI, AdhII and Ubi-1, wherein the promoter is operably linked to a polynucleotide comprising a polynucleotide sequence having at least 80% similarity to SEQ ID NO:
- 1 and coding for a dehydrin polypeptide having the amino acid sequence as shown in SEQ ID NO:
- 3,
- (b) mobilizing the recombinant construct into Agrobacterium strain to produce recombinant Agrobacterium strain,
- (c) obtaining an explant from the plant,
- (d) cultivating the explant with the recombinant Agrobacterium strain to produce transformed plant cells, and
- (e) culturing the transformed plant cells to produce transformed plants.
A transgenic plant transformed with a polynucleotide comprising a polynucleotide sequence having at least 80% similarity to SEQ ID NO:
- 1 and coding for a dehydrin polypeptide having the amino acid sequence as shown in SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.