Method of increasing yield in plants
AssigneesCropDesign N.V., Universidade Federal Do Rio De Janeiro
InventorsAdriana Silva Hemerly, Paulo Cavalcanti Gomes Ferreira, Stephane Rombauts
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Abstract
The present invention provides a method of increasing yield in plants, comprising transforming a plant with a nucleic acid encoding a CDC27 polypeptide or homolog thereof having a N-terminal deletion operably linked to a promoter active in the plant, where 100 to 220 amino acids are deleted at the N-terminus of the CDC27 polypeptide.
Claims10
A method for the production of a transgenic plant, plant cell or plant tissue comprising introduction into the genome of the plant, plant cell or plant tissue of a nucleic acid encoding a CDC27B polypeptide or homolog thereof having an N-terminal deletion, operably linked to a tissue-specific promoter, wherein 161 amino acids as represented by SEQ ID NO:
- 10 are deleted at the N-terminus of the CDC27B polypeptide as represented by SEQ ID NO:
- 13, or by a homolog thereof having at least 95% amino acid sequence identity to SEQ ID NO:
- 13
A plant cell transformed with a vector comprising a nucleic acid encoding a CDC27B polypeptide or homolog thereof having an N-terminal deletion operably linked to a tissue-specific promoter, wherein 161 amino acids as represented by SEQ ID NO:
- 10 are deleted at the N-terminus of the CDC27B polypeptide as represented by SEQ ID NO:
- 13, or by a homolog thereof having at least 95% amino acid sequence identity to SEQ ID NO:
- 13.
A method for modulating DNA replication in plant cells, plant parts or plants, comprising:
- (i) transforming one or more plant cells with a nucleic acid encoding a CDC27B polypeptide or homolog thereof having an N-terminal deletion operably linked to a tissue-specific promoter active in the shoot apical meristem of a plant, wherein 161 amino acids as represented by SEQ ID NO:
- 10 are deleted at the N-terminus of the CDC27B polypeptide as represented by SEQ ID NO:
- 13, or by a homolog thereof having at least 95% amino acid sequence identity to SEQ ID NO:
- 13;
- and
- (ii) maintaining or culturing the plants cells to regenerate plant parts or plants from the transformed cells;
- and
- (iii) incubating the cells, plant parts or plants under conditions allowing the expression of the nucleic acid of(i) to produce a CDC27B polypeptide or homolog thereof having an N-terminal deletion, wherein 161 amino acids as represented by SEQ ID NO:
- 10 are deleted at the N-terminus of the CDC27B polypeptide as represented by SEQ ID NO:
- 13, or by a homolog thereof having at least 95% amino acid sequence identity to SEQ ID NO:
- 13.
Dependencies are inferred from the claim text.