Fatty acid desaturases from fungi
AssigneeMonsanto Technology LLC
InventorsVirginia M. Ursin, Toni Voelker, Byron Froman
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Abstract
The invention relates generally to methods and compositions concerning fungal desaturase enzymes that modulate the number and location of double bonds in long chain poly-unsaturated fatty acids (LC-PUFA's). In Particular, the invention relates to methods and compositions for improving omega-3 fatty acid profiles in plant products and parts using desaturase enzymes and nucleic acids encoding for such enzymes. In particular embodiments, the desaturase enzymes are fungal −15 desaturases. Also provided are improved canola oil compositions having SDA and maintaining beneficial oleic acid content.
Claims32
An isolated polynucleotide comprising a nucleic acid sequence that encodes a polypeptide having desaturase activity that desaturates a fatty acid molecule at carbon 15, wherein the polynucleotide is selected from the group consisting of:
- (a) a polynucleotide encoding the polypeptide of SEQ ID NO:
- 3;
- (b) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 2;
- (c) a polynucleotide with at least 90% identity to SEQ ID NO:
- 1 or SEQ ID NO:
- 2;
- and
- (d) a polynucleotide encoding a polypeptide sequence with at least 90% identity to SEQ ID NO:3.
Dependencies are inferred from the claim text.