Expression control elements from the lemnaceae family
AssigneeBiolex, Inc.
InventorsLynn F. Dickey, Kevin M. Cox, Charles G. Peele
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AssigneeBiolex, Inc.
InventorsLynn F. Dickey, Kevin M. Cox, Charles G. Peele
Estimating the term…
Compositions and methods for regulating expression of nucleotide sequences of interest in a plant are provided. Compositions include novel nucleic acid molecules, and variants and fragments thereof, for expression control elements isolated from the Lemnaceae ubiquitin, r-histone and chitinase genes. A method for expressing a nucleotide sequence of interest in a plant using the expression control elements disclosed herein is further provided. The method includes introducing into a plant or plant cell or nodule an expression construct comprising an expression control element of the present invention operably linked to a nucleotide sequence of interest. In particular, the compositions and methods find use in enhancing expression of nucleotide sequences of interest in duckweed. Also provided is a novel Lemnaceae signal peptide-encoding sequence and the signal peptide encoded thereby. Where an expression construct of the invention is designed to express a polypeptide of interest, this novel signal peptide-encoding sequence can be included within the expression construct of the invention to provide for extracellular secretion of the encoded polypeptide of interest.
An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
An expression construct comprising an isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
A method for expressing a nucleotide sequence in a plant or plant cell or nodule, said method comprising introducing into the plant or plant cell or nodule an expression construct, said expression construct comprising an expression control element operably linked to a heterologous nucleotide sequence of interest, wherein said expression control element comprises a nucleotide sequence selected from the group consisting of:
Dependencies are inferred from the claim text.