Purification of a triple helix formation with an immobilized oligonucleotide
AssigneeCentelion
InventorsJoël Crouzet, Daniel Scherman, Pierre Wils
Life of this patent
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Abstract
A method for double-stranded DNA purification, by which a solution containing DNA in a mixture with other components is passed over a support on which is covalently coupled an oligonucleotide capable of hybridizing with a specific sequence present on the DNA to form a triple helix.
Claims2
A support comprising a covalently coupled oligonucleotide, wherein the oligonucleotide comprises a pyrimidine-rich sequence which forms a triple helix with a double-stranded DNA by hybridizing with a nucleotide sequence in the double-stranded DNA, wherein the oligonucleotide comprises the sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 18, or SEQ ID NO:
- 19.
A method of making a support for purifying double-stranded DNA, comprising covalently coupling an oligonucleotide to a support, wherein the oligonucleotide comprises the sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 18, or SEQ ID NO:
- 19.
Dependencies are inferred from the claim text.