Rapid isolation of osteoinductive protein mixtures from mammalian bone tissue
AssigneeZimmer Orthobiologics, Inc.
InventorsKevin Thorne, Rama Akella
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Abstract
A method for purifying bone-derived osteoinductive proteins including a demineralization process, a protein extraction process, a high molecular weight ultrafiltration process, a low molecular weight ultrafiltration process, and a recovery process. The high and low ultrafiltration processes preferably select proteins having a nominal molecular weight between approximately 8 kilodaltons and approximately 100 kilodaltons. Processes of the present invention may be used to recover osteoinductive proteins from bone demineralization waste streams.
Claims16
A process for obtaining osteoinductive proteins from mammalian bone tissue, comprising:
- contacting bone tissue with an acidic demineralization medium to provide demineralized bone tissue and a mineralized supernatant solution;
- separating the mineralized supernatant solution from the demineralized bone tissue;
- and removing at least some mineral from the mineralized supernatant solution by performing tangential flow filtration on the mineralized supernatant solution, to yield a demineralized supernatant solution containing osteoinductive proteins.
A process for obtaining osteoinductive proteins from mammalian bone tissue, comprising:
- contacting bone tissue with an acidic demineralization medium to provide demineralized bone tissue and a mineralized supernatant solution;
- separating the mineralized supernatant solution from the demineralized bone tissue;
- and removing at least some mineral from the mineralized supernatant solution by contacting the mineralized supernatant solution with a mineral precipitation agent, to yield a demineralized supernatant solution containing osteoinductive proteins.
Dependencies are inferred from the claim text.