Method of preparation of a fungal glucane hydrogel having antibacterial and immunostimulant activity, and use thereof

A method of preparing a fungal glucane hydrogel having antibacterial and immunostimulant activity, by the following steps:

• 1. alkaline deproteination of fruiting bodies of oyster mushroom (Pleurotus ostreatus);
• 2. subsequent elimination of water soluble components to obtain insoluble glucane;
• 3. followed by hydration and defibration of the insoluble glucane by wet grinding, wherein the wet grinding is carried out at a rotational speed of 3000-9000 rpm for 10-20 minutes and to a swelling volume in water of 50 to 500 ml/g.;
• 4. followed by heat sterilization at a temperature of 90 to 110° C. for 20 to 30 minutes, resulting in the hydrogel which is formed by the fungal polysaccharide from the fruiting body of Pleurotus ostreatus, with the β-(1,3)-D-glycosidic bond in the principal chain, in a concentration of 0.5 to 3% by weight.