US 7,622,301 B2 · Compositions and methods using RNA interference for control of nematodes

Abstract

The present invention concerns double stranded RNA compositions and transgenic plants capable of inhibiting expression of essential genes in parasitic nematodes, and methods associated therewith. Specifically, the invention relates to the use of RNA interference to inhibit expression of a target essential parasitic nematode gene selected from the group consisting of a parasitic nematode cytosolic chaperonin gene, a parasitic nematode gene encoding heat shock protein-90, a parasitic nematode gene homologous to the C. elegans Y65B4BR.5a gene, and a parasitic nematode gene homologous to a C. elegans pat-10 gene, and relates to the generation of plants that have increased tolerance to parasitic nematodes.

Claims20

Dependent claims

A transgenic plant capable of expressing a dsRNA comprising

i) a first strand which comprises a fragment of at least 19 contiguous nucleotides of SEQ ID NO:5, SEQ ID NO:6, or SEQ ID NO:7;
and
ii) a second strand that is completely complementary to the first strand.

A method of making a transgenic plant capable of expressing a dsRNA comprising a first strand that is identical to a portion of a pat-10 target gene in a parasitic nematode and a second strand that is complementary to the first strand, said method comprising the steps of:

a) preparing a nucleic acid sequence comprising a fragment of at least 19 contiguous nucleotides of SEQ ID NO :5, SEQ ID NO:6, or SEQ ID NO:7 wherein the nucleic acid sequence is able to form a double-stranded transcript once expressed in the plant;
b) contacting a recipient plant with said nucleic acid;
c) producing one or more offspring of said recipient plant;
and
d) testing the offspring for expression of said double-stranded transcript.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

10/906,472Filed 22 Feb 2005 · us provisional application

Priority

24 Feb 2004

Published

US 2005/0188438 A125 Aug 2005

Granted

24 Nov 2009

Term ends

Not estimatedThe source records don’t support an estimate.

Classification5 codes

C12N 15/8218
Methods for controlling, regulating or enhancing expression of transgenes in plant cells: antisense, co-suppression, viral induced gene silencing…
C12N 15/8285
For biotic stress resistance, pathogen resistance, disease resistance: for nematode resistance
Y02A 40/146
In agriculture: genetically Modified [GMO] plants, e.g. transgenic plants
C12N
Microorganisms or enzymes

Compositions and methods using RNA interference for control of nematodes

Life of this patent

Compositions and methods using RNA interference for control of nematodes

Life of this patent

Abstract

Claims20