Agarase and gene thereof
AssigneeTakara Bio Inc.
InventorsJun Tomono, Hiroaki Sagawa, Ikunoshin Kato
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Abstract
Production of efficiently producing agaro-oligosaccharide; a polypeptide having an agarase activity which is usable in, for example, efficiently extracting a substance such as a nucleic acid from an agarose gel; the amino acid sequence of the polypeptide; a gene encoding the polypeptide; a process for producing the polypeptide; a process for producing agaro-oligosaccharide; and a process for extracting a substance such as a nucleic acid from an agarose gel.
Claims17
An isolated agarase which comprises the amino acid sequence of 417 residues from residue 21 to residue 437 in the amino acid sequence of SEQ ID NO:22, or an amino acid sequence that is encoded by a DNA which hybridizes to the nucleotide sequence of 1251 nucleotides from nucleotide 61 to nucleotide 1311 in the nucleotide sequence of SEQ ID NO:20 in a solution containing 6×SSC (1×SSC:
- 0.15 M NaCl, 0.015 M sodium citrate, pH 7.0), 0.5% SDS, 5×Denhardt's and 100 mg/ml herring sperm DNA at 65° C. and has an activity of hydrolyzing a β-1,4 bond between D-galactose and 3,6-anhydro-L-galactose in agarose.
An isolated gene which hybridizes to a gene encoding the agarase which comprises the amino acid sequence of 417 residues from residue 21 to residue 437 in the amino acid sequence of SEQ ID NO:22 in a solution containing 6×SSC (1×SSC:
- 0.15M NaCl, 0.015M sodium citrate, pH 7.0), 0.5% SDS, 5×Denhardt's and 100 mg/ml herring sperm DNA at 65° C. and which encodes an agarase having an activity of hydrolyzing a β-1,4 bond between D-galactose and 3,6-anhydro-L-galactose in agarose.
A recombinant DNA molecule which comprises a gene selected from the group consisting of:
- (a) a gene encoding an agarase which comprises the amino acid sequence of 417 residues from residue 21 to residue 437 in the amino acid sequence of SEQ ID NO:22, and has an activity of hydrolyzing a β-1,4 bond between D-galactose and 3,6-anhydro-L-galactose in agarose;
- and
- (b) a gene which hybridizes to the gene of (a) in a solution containing 6×SSC (1×SSC:
- 0.15 M NaCl, 0.015 M sodium citrate, pH 7.0), 0.5% SDS, 5×Denhardt's and 100 mg/ml herring sperm DNA at 65° C. and encodes an agarase having an activity of hydrolyzing a β-1,4 bond between D-galactose and 3,6-anhydro-L-galactose in agarose.
Dependencies are inferred from the claim text.