Myceliophthora thermophila laccase variants
AssigneeNovozymes A/S
InventorsAllan Svendsen, Feng Xu
Life of this patent
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Abstract
The present invention relates to a method of designing laccase mutants with increased oxidation potential and/or changed pH optimum and/or altered mediator pathway and/or altered O2/OH−-pathway, which method is based on the hitherto unknown three dimensional structure of laccases, wherein the parent laccase is a Myceliophthora thermophila, Polyporus pinsitus, or Coprinus cinereus laccase.
Claims33
An isolated variant of a laccase, wherein the variant has laccase activity and has the amino acid sequence of SEQ ID NO:
- 10 except for one or more of the following mutations:
- (a) H93E;
- (b) H95E;
- (c) D106A, D106F, D106I, D106L, D106P, D106V, D106W, or D106Y;
- (d) A108F, A108I, A108L, A108P, A108W, or A108Y;
- (e) N109A, N109D, N109F, N109I, N109L, N109P, N109Q, N109V, N109W, or N109Y;
- (f) T428A, T428F, T428I, T428L, T428P, T428V, T428W, or T428Y;
- (g) L500F, L500I, L500W, or L500Y;
- (h) A506E;
- (i) S510A, S510F, S510I, S510L, S510P, S510V, S510W, or S510Y;
- (j) G511F, G511I, G511L, G511P, G511V, G511W, or G511Y;
- or
- (k) G514A, G514F, G514I, G514L, G514P, G514V, G514W, or G514Y.
An isolated variant of a laccase, wherein the variant has laccase activity and has the amino acid sequence of SEQ ID NO:
- 10 except for a substitution at one or more of the following positions:
- 185, 187, 189, 191, 193, 234, 235, 236, 269, 293, and 294.
An isolated variant of a laccase, wherein the variant has laccase activity and has the amino acid sequence of SEQ ID NO:
- 10 except for a substitution at one or more of the following positions:
- 185, 187, 189, 191, 193, 234, 235, 236, 269, 293, and 294;
- and
- a substitution at one or more of the following positions:
- 186, 188, 190, 192, and 194.
Dependencies are inferred from the claim text.