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US 7,622,280 B2Granted
Emulsion compositions
Assignee454 Life Sciences Corporation
InventorsPhillip Holliger, Farid Ghadessy
Life of this patent
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Abstract
An emulsion is useful in allowing a wide variety of gene products to be expressed via eukaryotic in vitro expression. The emulsion comprises a silicone based surfactant, a hydrophobic phase and a hydrophilic phase; wherein the hydrophilic phase comprises a plurality of compartments containing a functional in vitro eukaryotic expression system.
Claims12
A method for increasing the concentration of a nucleic acid molecule comprising the steps of:
- (a) forming microcapsules from a water-in-oil emulsion consisting of:
- a chemically inert silicone-based surfactant, a hydrophobic oil external phase, and a hydrophilic aqueous internal phase comprising the microcapsules, wherein the microcapsules include a nucleic acid molecule, a bead capable of being linked to the nucleic acid molecule, and an aqueous solution comprising components necessary to perform nucleic acid amplification;
- (b) amplifying the nucleic acid molecule in the microcapsules to form amplified copies of the nucleic acid molecule, wherein the amplification employs a thermal cycling process, thereby increasing the concentration of the nucleic acid molecule in the microcapsules.
Dependencies are inferred from the claim text.