Methods of screening tyramine- and octopamine-expressing cells for compounds and compositions having potential insect control activity
AssigneeTyratech, Inc.
InventorEssam Enan
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Abstract
A screening method for identifying compounds that are effective insect control agents includes providing cells expressing an octopamine receptor, adding the compounds to the cells, and measuring the effects of the compounds and compositions. The effects of the compounds may be determined by measuring the binding affinity of the compounds to the octopamine receptor or measuring the change in intracellular cAMP or Ca2+ levels.
Claims18
A method of screening compounds and/or compositions for potential insect control activity, comprising:
- providing cells expressing an octopamine receptor and cells expressing a tyramine receptor;
- adding said compounds and/or compositions to the cells thereb causing an effect through at least the tyramine receptor;
- measuring the effects of the compounds and/or compositions, wherein measurable effects comprise a change in a level of cAMP and/or Ca2+within the cells, and wherein the effects are indicative of potential insect control activity. classifying the selected compounds and/or compositions as having potential insect control activity.
A method of screening compounds and/or compositions for potential insect control activity, comprising:
- providing first cells expressing a first octopamine receptor and cells expressing a tyramine receptor;
- providing second cells expressing a second octopamine receptor;
- adding said compounds and/or compositions to the first and the second cells thereby causing an effect through at least the tyramine receptor;
- measuring the effects of the compounds and/or compositions, wherein measurable effects comprise a change in a level of cAMP and/or Ca2+ within the cells, and wherein the effects are indicative of potential insect control activity. classifying the selected compounds and/or compositions as having potential insect control activity.
A method of testing the effects of compounds and/or compositions on cells, said method comprising:
- providing first cells expressing a first octopamine receptor cloned from a first insect species-of-interest and a tyramine receptor;
- providing second cells expressing a second octopamine receptor cloned from a second insect species-of-interest;
- adding said compounds and/or compositions to the first and the second cells thereby causing an effect through at least the tyramine receptor;
- measuring the effects of the compounds and/or compositions, wherein measurable effects comprise a change in a level of cAMP and/or Ca2+ within the cells, and wherein the effects are indicative of potential insect control activity
- classifying the selected compound and/or compositions as having potential insect control activity.
Dependencies are inferred from the claim text.