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AssigneeCanon Kabushiki Kaisha
InventorsKazuhiro Ban, Hidenori Shiotsuka, Takeshi Imamura
Estimating the term…
To provide an organic substance-immobilized structure employing a novel immobilizing technique and a manufacturing method thereof using the novel immobilizing technique, where, when an organic substance, particularly a biological substance is immobilized on the surface of a substrate, the organic substance, particularly the biological substance can be stably immobilized on the surface of the substrate through orientation of the organic substance, particularly the biological substance suitable for exerting physiological functions thereof. At least part of the substrate's surface is constructed of one or more substrates containing aluminum oxide. The immobilization of the organic substance to the surface of the substrate is carried out by binding at least part of the binding domain to the surface of the substrate through a binding domain containing a peptide having an affinity to aluminum oxide and composed of at least one or more amino acids, which is coupled with the organic substance.
A method of manufacturing a structure which comprises a protein and a substrate comprising the steps of:
Dependencies are inferred from the claim text.
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With an insoluble carrier for immobilising immunochemicals: with ligand attached to the carrier via a chemical coupling agent
With an insoluble carrier for immobilising immunochemicals: the carrier being inorganic
Investigating or analysing materials by determining their chemical or physical properties
Term ended 19 Apr 2026 (estimated)
The present invention relates to an organic substance-immobilized substrate prepared by immobilizing an organic substance on a substrate's surface at least part of which has an aluminum oxide layer and to a method of manufacturing the same, and further relates to a peptide having an affinity to a layer containing aluminum oxide and used for immobilization of the organic substance and to a DNA that encodes the peptide having the affinity. More particularly, the present invention relates to a biological substance-immobilized substrate which is applicable to a target substance-detecting element, a target substance-converting element, a target substance-separating element, and a protein structure-optimizing element by the utilization of a biological substance immobilized on the substrate, and to a manufacturing method therefor.
Many studies and developments have been conducted for a wide range of applications of so-called biosensors and bioreactors that utilize molecular recognition ability and substance-converting ability of biological substances such as enzymes and antibodies as well as nucleic acid molecules (e.g., DNA and RNA) including genes.
For the biosensors, there are growing demands on further technical developments for applications on various detection targets in conjunction with growing interests in health as well as matters of environmental pollutants and public safety thereof. Recently, furthermore, the bioreactors have attracted much attention as eco-friendly clean processing technologies. Therefore, for example, there are increasing demands on further technical developments such as those in processes of producing products utilizing various bioprocesses.
FIG. 1 is a cross sectional diagram schematically showing a configuration of a structure of an example of an organic substance-immobilized structure according to the present invention;
FIG. 2 is a cross sectional diagram schematically showing the configuration of the structure of an example of the organic substance-immobilized structure according to the present invention;
FIG. 3 is a cross sectional diagram schematically showing the configuration of the structure of an example of the organic substance-immobilized structure according to the present invention;
The organic substance-immobilized structure of the present invention is a structure where an organic substance is immobilized on the surface of the substrate, characterized by including: a substrate having a surface at least part of which contains aluminum oxide; and an organic substance being immobilized on the surface of the substrate through a binding domain containing a peptide composed of at least one or more amino acids. In particular, the peptide included in the binding domain is characterized by containing an amino acid sequence having an ability to bind to the aluminum oxide.
On the other hand, the organic substance may include a biological substance and the biological substance may include a protein or a part thereof. At this time, in a coupling portion between the biological substance and the binding domain, a linker composed of one or more amino acids may be included between a peptide containing an amino acid sequence having an ability to bind to the aluminum oxide in the binding domain and the biological substance.
The peptide, which contains the amino acid sequence having the ability to bind to the aluminum oxide in the binding domain, is a peptide containing the whole of at least one amino acid sequence selected from the group consisting of SEQ ID NOS: 1 to 32 described below, or an amino acid sequence having a part thereof, or a peptide having a repetitive structure of any of these amino acid sequences, or a peptide containing a complex composed of these amino acid sequences, and alternatively the peptide may contain one or more sequences of the amino acid sequences of SEQ ID NOS: 30 to 32 and may form a cyclic structure with an intramolecular disulfide binding in the sequence.