Method for the diagnosis of cancers by measuring the changes of glycosylation of proteins related to tumorigenesis and metastasis and kit for diagnosis of cancers using the same

A method for diagnosing colon cancer by measuring extent of β1,6 N-acetylglucosamine glycosylation and expression of prostate-derived factor (PDF) protein related to tumorigenesis and metastasis, comprising:

• adhering antibodies against said PDF protein to two matrices, wherein one matrix is for analyzing expression of said PDF protein and the other is for analyzing extent of β1,6 N-acetylglucosamine glycosylation of said same PDF protein;
• adding a blood or serum sample from a patient to the two matrices to induce a first reaction;
• washing the matrices;
• one
• adding biotin-labeled antibodies against said PDF protein to the one matrix for analyzing expression of said PDF protein and adding biotin-labeled L4-PHA to the other matrix for analyzing extent of β1,6 N-acetylglucosamine glycosylation of said same PDF protein to induce a second reaction;
• washing the matrices;
• adding a coloring enzyme- or fluorescent substance-conjugated avidin to both matrices to induce a third reaction;
• washing the matrices;
• measuring optical density or detecting fluorescence;
• and
• comparing the expression of said PDF protein and the extent of β1,6 N-acetylglucosamine glycosylation of said same PDF protein between the blood or serum sample and a control sample, wherein an increase in the expression of said PDF protein and the extent of β1,6 N-acetylglucosamine glycosylation of said same PDF protein in the blood or serum sample relative to the control sample indicates the presence of colon cancer.