Ribozymes with broad tRNA aminoacylation activity
AssigneeThe Research Foundation of State University of New York
InventorsHiroaki Suga, Hiroshi Murakami, Hirohide Saito
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Abstract
The present invention provides catalytic RNA molecules having cis or trans aminoacylation activity. The catalytic RNA molecules having cis aminoacylation activity comprise a catalytic domain and an aminoacylation domain. The catalytic RNA molecules having trans aminoacylation activity only have the catalytic domain. A method is provided for constructing and screening of these molecules. These molecules are suitable for aminoacylating with specific amino acids.
Claims20
A polynucleotide comprising a sequence encoding a ribozyme, said ribozyme being capable of aminoacylating a tRNA with a non-cognate amino acid, wherein the sequence encoding the ribozyme is selected from the group consisting of SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, and SEQ ID NO:32.
A method of aminoacylating a tRNA with a non-cognate amino acid comprising the steps of:
- a) providing a ribozyme capable of aminoacylating tRNA with a non-cognate amino acid;
- wherein the ribozyme has a nucleotide sequence selected from the group consisting of SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, and SEQ ID NO:32:
- i) preparing a mixture comprising;
- b) providing a tRNA;
- c) providing a non-cognate amino acid;
- and
- d) combining a), b) and c) to effect aminoacylation of the tRNA with the non-cognate amino acid.
Dependencies are inferred from the claim text.