Monoclonal antibodies that bind or neutralize dengue virus
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsChing-Juh Lai, Robert H. Purcell
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Abstract
The present invention relates to monoclonal antibodies that bind or neutralize dengue type 1, 2, 3, and/or 4 virus. The invention provides such antibodies, fragments of such antibodies retaining dengue virus-binding ability, fully human or humanized antibodies retaining dengue virus-binding ability, and pharmaceutical compositions including such antibodies. The invention further provides for isolated nucleic acids encoding the antibodies of the invention and host cells transformed therewith. Additionally, the invention provides for prophylactic, therapeutic, and diagnostic methods employing the antibodies and nucleic acids of the invention.
Claims26
A monoclonal antibody, or binding fragment thereof that binds dengue type 4 virus, comprising:
- a heavy chain CDR1 polypeptide having the amino acid sequence of SEQ ID NO:
- 3, a heavy chain CDR2 polypeptide having the amino acid sequence of SEQ ID NO:
- 5 and a heavy chain CDR3 polypeptide having the amino acid sequence of SEQ ID NO:
- 7;
- and
- a light chain CDR1 polypeptide having the amino acid sequence of SEQ ID NO:
- 11, a light chain CDR2 polypeptide having the amino acid sequence of SEQ ID NO:
- 13 and a light chain CDR3 polypeptide having the amino acid sequence of SEQ ID NO:
- 15.
An isolated nucleic acid molecule having a nucleotide sequence encoding a heavy chain polypeptide comprising SEQ ID NO:1.
An isolated nucleic acid molecule having a nucleotide sequence encoding a light chain polypeptide comprising SEQ ID NO:9.
Humanized IgG1 5H2 plasmid deposited with ATCC as ATCC Accession No. PTA-5662.
An isolated humanized chimpanzee monoclonal antibody that neutralizes dengue virus, wherein said antibody is produced by a plasmid having ATCC Accession No. PTA-5662.
A substantially pure antibody comprising
- SEQ ID NO.:
- 3, SEQ ID NO.:
- 5, SEQ ID NO.:
- 7, SEQ ID NO.:
- 11, SEQ ID NO.:
- 13, and SEQ ID NO.:
- 15.
A monoclonal antibody or binding fragment thereof that binds to dengue type 4 virus, wherein said antibody or binding fragment comprises a heavy chain polypeptide having the amino acid sequence of SEQ ID NO:
- 1 and a light chain polypeptide having the amino acid sequence of SEQ ID NO:9.
Dependencies are inferred from the claim text.