Recombinant intracellular pathogen immunogenic compositions and methods for use
AssigneeThe Regents of the University of California
InventorsMarcus A. Horwitz, Gunter Harth
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Abstract
Immunogenic compositions comprising recombinant attenuated intracellular pathogens that have been transformed to express recombinant immunogenic antigens of the same or other intracellular pathogens are provided. Exemplary immunogenic compositions include, but are not limited to attenuated recombinant Mycobacteria expressing the major extracellular non-fusion proteins of Mycobacteria and/or other intracellular pathogens. Other embodiments are provided wherein the recombinant attenuated intracellular pathogen is auxotrophic.
Claims11
A prime-boost vaccine strategy for protecting a mammal against infection by a pathogen of the genus Mycobacterium comprising:
- administering a first priming immunogenic composition to a vaccinee wherein said first priming immunogenic composition is a Bacille Calmette Guèrin (BCG);
- and
- administering a second boosting immunogenic composition, after the passage of a period of time, to said vaccinee optionally in the presence of an adjuvant, wherein said second boosting immunogenic composition comprises at least one purified Mycobacteria major extracellular protein selected from the group consisting of Mycobacterium tuberculosis (Mtb) 23.5 kDa protein, Mtb 30 kDa protein (Ag85B), Mycobacterium bovis (MB) 30 kDa protein, Mycobacterium leprae (ML) 23.5 kDa protein, and ML 30 kDa protein;
- wherein a protective immune response against said pathogen of the genus Mycobacterium is produced in said vaccinee.
A prime-boost vaccine strategy for protecting a mammal against infection by a pathogen of the genus Mycobacterium comprising:
- administering a first priming immunogenic composition to a vaccinee wherein said first immunogenic priming composition is BCG;
- and
- administering a second boosting immunogenic composition, after the passage of a period of time, to said vaccinee wherein said second boosting immunogenic composition is purified Mycobacterium tuberculosis 30 kDa protein (Ag85B);
- wherein a protective immune response against said pathogen of the genus Mycobacterium is produced in said vaccinee.
A prime-boost vaccine strategy for protecting against infection by a pathogen of the genus Mycobacterium comprising:
- identifying an individual who has previously been immunized with BCG;
- and
- administering to said individual a boosting immunogenic composition, optionally in the presence of an adjuvant, wherein said boosting immunogenic composition comprises at least one purified Mycobacteria major extracellular protein selected from the group consisting of Mtb 23.5 kDa protein, Mtb 30 kDa protein (Aq85B), MB 30 kDa protein, ML 23.5 kDa protein, and ML 30 kDa protein;
- wherein a protective immune response against said pathogen of the genus Mycobacterium is produced in said individual.
Dependencies are inferred from the claim text.