Solution phase electrophoresis device, components, and methods

A device for solution phase electrophoretic separation of analytes within a sample, comprising:

• an anode within an anode buffer chamber;
• a cathode within a cathode buffer chamber;
• a chamber stack disposed between said anode and said cathode;
• and
• chamber stacking means external to said chamber stack;
• wherein said chamber stack comprises a plurality of detachably mated sample chambers having lumens aligned along the electrical axis between said anode and cathode, the lumens of said coaxially aligned sample chambers being collectively capable of defining an electrically conductive fluid column therethrough;
• wherein said chamber stack further comprises a plurality of junctional partitions, each of said partitions positioned at a different one of the junctions between adjacent sample chambers, said partitions being permeable to ions and to at least a plurality of the analytes in said sample;
• and
• wherein said chamber stacking means comprises a loading tube and a removable end cap that engages said loading tube to secure said chamber stack, wherein said end cap comprises a lumen that permits fluid communication between the interior of said chamber stack and said cathode buffer chamber.