Methods and compositions for detection of microbial contaminants in peritoneal dialysis solution

A method of providing dialysis to a patient, the method comprising:

• preparing a dialysis solution including:
• a) providing a glucose polymer in a powder form;
• b) performing a modified bioburden test on the glucose polymer to detect contaminating Alcyclobacillus acidocaldarius;
• c) preparing a glucose polymer solution using the powder form of the glucose polymer;
• d) sterilizing the glucose polymer solution;
• e) adding a reagent derived from silkworm larvae plasma to the sterilized glucose polymer solution, wherein the reagent is reactive with peptidoglycan to determine a peptidoglycan concentration;
• and
• f) using the sterilized glucose polymer solution to prepare the dialysis solution if the peptidoglycan concentration is determined to be about 10 ng/mL or less;
• and
• providing the dialysis solution to the patient.