# US 7,612,256 B2 · Isolated nucleic acid molecules encoding the Br2 P-glycoprotein of maize and methods of modifying growth in plants transformed therewith

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- Number: US 7,612,256 B2
- Document ID: US7612256B2
- Status: Granted
- Kind: B2
- Application number: 11/338,356
- Filed: 2006-01-24
- Published: 2007-04-05
- Granted: 2009-11-03
- Earliest claimed: 1999-11-12
- Assignees: Pioneer Hi-Bred International, Inc.; The Curators of the University of Missouri
- Inventors: Gurmukh S. Johal; Dilbag S. Multani; Steven P. Briggs
- Web page: https://patentgrep.com/patent/US7612256B2

## CPC

- C07K 14/415: from plants
- C12N 15/8261: {with agronomic (input) traits, e.g. crop yield}
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The invention relates to the genetic modification of plants, particularly to the expression of P-glycoprotein genes in transformed plants. Nucleotide sequences for the Br2 gene encoding a P-glycoprotein of maize and methods for their use are provided. The sequences find use in modifying the growth of plants.

## Claims

### Claim 1

An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO: 1;
(b) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO: 2;
(c) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO: 3, wherein said variant has at least 90% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO: 3; and
(d) a nucleotide sequence that is fully complementary to the nucleotide sequence of any one of (a)-(c);
wherein said nucleotide sequence encodes a P-glycoprotein that controls plant height or said nucleotide sequence is complementary to a nucleotide sequence that encodes said P-glycoprotein.

### Claim 2

An expression cassette comprising a nucleic acid molecule operably linked to a promoter that drives expression in a plant cell, wherein said nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO: 1;
(b) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO: 2;
(c) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO: 3, wherein said variant has at least 90% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO: 3; and
(d) a nucleotide sequence that is fully complementary to the nucleotide sequence of any one of (a)-(c);
wherein said nucleotide sequence encodes a P-glycoprotein that controls plant height or said nucleotide sequence is complementary to a nucleotide sequence that encodes said P-glycoprotein.

### Claim 3 (depends on 2)

The expression cassette of claim 2, wherein said promoter is selected from the group consisting of tissue-preferred, stem-preferred, constitutive, chemically regulatable, and pathogen-inducible promoters.

### Claim 4

A transformed plant cell having stably incorporated into its genome a nucleic acid molecule operably linked to a promoter that drives expression in a plant cell, wherein said nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO: 1;
(b) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO: 2;
(c) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO: 3, wherein said variant has at least 90% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO: 3; and
(d) a nucleotide sequence that is fully complementary to the nucleotide sequence of any one of (a)-(c);
wherein said nucleotide sequence encodes a P-glycoprotein that controls plant height or said nucleotide sequence is complementary to a nucleotide sequence that encodes said P-glycoprotein.

### Claim 5

A transformed plant having stably incorporated into its genome a nucleic acid molecule operably linked to a promoter that drives expression in a plant cell, wherein said nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO: 1;
(b) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO: 2;
(c) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO: 3, wherein said variant has at least 90% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO: 3; and
(d) a nucleotide sequence that is fully complementary to the nucleotide sequence of any one of (a)-(c);
wherein said nucleotide sequence encodes a P-glycoprotein that controls plant height or said nucleotide sequence is complementary to a nucleotide sequence that encodes said P-glycoprotein.

### Claim 6 (depends on 5)

The plant of claim 5, wherein said promoter is selected from the group consisting of tissue-preferred, stem-preferred, constitutive, chemically regulatable, and pathogen-inducible promoters.

### Claim 7 (depends on 5)

The plant of claim 5, wherein said plant is a monocot or a dicot.

### Claim 8 (depends on 7)

The plant of claim 7, wherein said monocot is selected from the group consisting of maize, wheat, rice, sorghum, rye, millet and barley.

### Claim 9 (depends on 8)

The plant of claim 8, wherein said rice is Basmati rice.

### Claim 10 (depends on 7)

The plant of claim 7, wherein said dicot is selected from the group consisting of soybeans, sunflowers, safflowers, alfalfa, Brassica sp., cotton, peanuts and fruit trees.

### Claim 11

A transformed seed of a transformed plant, said transformed plant having stably incorporated into its genome a nucleic acid molecule operably linked to a promoter that drives expression in a plant cell, wherein said nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO: 1;
(b) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO: 2;
(c) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO: 3, wherein said variant has at least 90% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO: 3; and
(d) a nucleotide sequence that is fully complementary to the nucleotide sequence of any one of (a)-(c);
wherein said nucleotide sequence encodes a P-glycoprotein that controls plant height or said nucleotide sequence is complementary to a nucleotide sequence that encodes said P-glycoprotein and wherein said seed comprises said nucleic acid molecule operably linked to said promoter.

### Claim 12

A method for modifying the growth of a plant, said method comprising transforming a plant with a nucleic acid molecule operably linked to a promoter that drives the expression of said nucleic acid molecule in said plant, wherein said nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO: 1;
(b) a nucleotide sequence having at least 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO: 2;
(c) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO: 3, wherein said variant has at least 90% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO: 3; and
(d) a nucleotide sequence that is fully complementary to the nucleotide sequence of any one of (a)-(c);
wherein said nucleotide sequence encodes a P-glycoprotein that controls plant height or said nucleotide sequence is complementary to a nucleotide sequence that encodes said P-glycoprotein and wherein the height of the transformed plant is reduced or increased when compared to an untransformed plant.

### Claim 13 (depends on 12)

The method of claim 12, wherein said promoter is selected from the group consisting of tissue-preferred, stem-preferred, constitutive, chemically regulatable, and pathogen-inducible promoters.