# US 7,612,255 B2 · Transgenic plants for mitigating introgression of genetically engineered genetic traits

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- Number: US 7,612,255 B2
- Document ID: US7612255B2
- Status: Granted
- Kind: B2
- Application number: 10/774,388
- Filed: 2004-02-10
- Published: 2004-09-02
- Granted: 2009-11-03
- Earliest claimed: 1999-02-03
- Inventors: Jonathan Gressel; Hani Al-Ahmad
- Web page: https://patentgrep.com/patent/US7612255B2

## CPC

- C12N 15/8241: {Phenotypically and genetically modified plants via recombinant DNA technology}
- C12N 15/8265: {Transgene containment, e.g. gene dispersal}
- C12N 15/8287: {for fertility modification, e.g. apomixis}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Genetic mechanisms for mitigating the effects of introgression of a genetically engineered genetic trait of a cultivated crop to an undesirable, interbreeding related species.

## Claims

### Claim 1

A method to provide a mitigation to effects of introgression of at least one advantageous genetically engineered trait to an uncultivated interbreeding species related to the transformed cultivated crop, the method comprising transforming a population of plants of the cultivated crop to co-express the at least one advantageous genetically engineered trait, and at least one mitigating genetic trait, wherein:
said at least one advantageous genetic trait is encoded by an advantageous gene conferring a trait selected from the group consisting of herbicide resistance, disease, insect and nematode resistance, environmental stress resistance, high productivity, modified agronomic quality, enhanced yield, modified ripening, bioremediation, expression of heterologous products and genetically modified plant products;
said at least one mitigating genetic trait is encoded by a mitigating gene, said mitigating gene being an anti-shattering gene, an anti-bolting gene or a dwarfism gene;
said advantageous gene and said mitigating gene having a genetic distance of no greater than 10 centimorgans from each other so as to produce tandem introgression of said advantageous and said mitigating traits into said uncultivated interbreeding species; and
wherein introgression and expression of said mitigating genetic trait in said uncultivated interbreeding species related to the cultivated crop renders said uncultivated interbreeding species less fit compared to a similar uncultivated interbreeding species related to the cultivated crop and not expressing said mitigating genetic trait.

### Claim 2 (depends on 1)

The method of claim 1, wherein said at least one mitigating genetic trait is an endogenous genetic trait of said cultivated crop.

### Claim 3 (depends on 1)

The method of claim 1, wherein said cultivated crop is tobacco, rice or oilseed rape, said advantageous genetic trait is herbicide resistance, and said mitigating genetic trait is gibberellic acid insensitivity.

### Claim 4 (depends on 1)

The method of claim 1, wherein said cultivated crop is sugarbeet, said advantageous genetic trait is herbicide resistance, and said mitigating genetic trait is antibolting.

### Claim 5 (depends on 1)

The method of claim 1, wherein said advantageous genetic trait is herbicide resistance, and said mitigating genetic trait is anti-seed shattering.

### Claim 6 (depends on 5)

The method of claim 5, wherein said cultivated crop is selected from the group consisting of tobacco, rice and oilseed rape.

### Claim 7 (depends on 1)

The method of claim 1, wherein said cultivated crop is selected from the group consisting of tobacco, rice and oilseed rape.

### Claim 8 (depends on 1)

The method of claim 1, wherein said herbicide resistance is conferred by a ahasR (acetohydroxy acid synthase) gene.

### Claim 9 (depends on 1)

The method of claim 1, wherein said dwarfism gene is a Δgai (gibberellic acid-insensitive) mutant gene and said anti-shattering gene is a shatterproof gene.

### Claim 10

A method to provide a mitigation to effects of introgression of an advantageous genetically engineered trait to an uncultivated interbreeding species related to the transformed cultivated crop, the method comprising transforming a population of plants of the cultivated crop to co-express the advantageous genetically engineered trait, and a mitigating genetic trait, wherein:
said advantageous genetic trait is encoded by an advantageous gene encoding a protein conferring herbicide resistance;
said mitigating genetic trait is encoded by an anti-shattering gene;
said advantageous gene and said anti-shattering gene having a genetic distance of no greater than 10 centimorgans from each other so as to produce tandem introgression of said advantageous and said mitigating traits into said uncultivated interbreeding species; and
wherein introgression and expression of said mitigating genetic trait in said uncultivated interbreeding species related to the cultivated crop renders said uncultivated interbreeding species less fit compared to a similar uncultivated interbreeding species related to the cultivated crop and not expressing said mitigating genetic trait.

### Claim 11 (depends on 1)

The method according to claim 1, wherein sequences of the advantageous gene and the mitigating gene are juxtaposed.