# US 7,612,198 B2 · Soluble highly branched glucose polymers

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- Number: US 7,612,198 B2
- Document ID: US7612198B2
- Status: Granted
- Kind: B2
- Application number: 11/015,640
- Filed: 2004-12-20
- Published: 2005-07-21
- Granted: 2009-11-03
- Earliest claimed: 2003-12-19
- Assignees: Roquette Freres
- Inventors: Patrick Fuertes; Jean-Michel Roturier; Carole Petitjean
- Web page: https://patentgrep.com/patent/US7612198B2

## CPC

- C12P 19/04: Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- A61P 3/00: Drugs for disorders of the metabolism (of the blood or the extracellular fluid A61P7/00)
- A61P 3/10: for hyperglycaemia, e.g. antidiabetics
- C08B 30/12: Degraded, {destructured} or non-chemically modified starch {, e.g. mechanically, enzymatically or by irradiation; Bleaching of starch (preparation of chemical derivatives of starch C08B31/00)}
- C08B 30/18: Dextrin {, e.g. yellow canari, white dextrin, amylodextrin or maltodextrin; Methods of depolymerisation, e.g. by irradiation or mechanically}
- C08B 30/20: Amylose or amylopectin (chemical derivatives thereof C08B33/00, C08B35/00)
- C08B 35/00: Preparation of derivatives of amylopectin
- C12P 19/16: produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin (non-biological hydrolysis of starch C08B30/00)
- C12P 19/22: produced by the action of a beta-amylase, e.g. maltose
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C08B: POLYSACCHARIDES; DERIVATIVES THEREOF (polysaccharides containing less than six saccharide radicals attached to each other by glycosidic linkages C07H; fermentation or enzyme-using processes C12P19/00; sugar industry C13; production of cellulose D21)

## Abstract

The invention relates to soluble highly branched glucose polymers, having a reducing sugar content of less than 1%, a level of α-1,6 glucoside bonds of between 13 and 17% and an Mw having a value of between 0.9×105 and 1.5×105 daltons, characterized in that their branched chain length distribution profile consists of 70 to 85% of DP of less than 15, of 10 to 16% of DP of between 15 and 25 and of 8 to 13% of DP greater than 25.

## Claims

### Claim 1

Soluble highly branched glucose polymers, having a reducing sugar content of less than 1%, a level of α-1,6 glucoside bonds of between 13 and 17% and a molecular weight having a value of between 0.9×105 and 1.5×105 daltons, wherein the soluble highly branched glucose polymers have a branched chain length distribution profile of 70 to 85% of DP of less than 15, of 10 to 14% of DP of between 15 and 25 and of 8 to 13% of DP greater than 25.

### Claim 2 (depends on 1)

The polymers according to claim 1, wherein said soluble highly branched glucose polymers have an intrinsic coefficient of viscosity “a” according to the MARK, HOUWINK and SAKURADA equation of less than or equal to 0.1.

### Claim 3 (depends on 1)

The polymers according to claim 1, wherein said soluble highly branched glucose polymers have between at least 12% and at most 14% with a DP of between 15 and 25.

### Claim 4 (depends on 1)

The polymers according to claim 1, wherein said soluble highly branched glucose polymers have between at least 10% and at most 12% with a DP of between 15 and 25.

### Claim 5 (depends on 1)

A method for preparing the soluble highly branched glucose polymers according to claim 1, comprising:
i) preparing an aqueous starch solution having an amylose content of at least 30% by weight,
ii) treating the said solution with a branching enzyme and then a β-amylase successively,
iii) carrying out a fractionation so as to recover the high molecular weight fractions, and
iv) collecting the highly branched glucose polymers thus obtained.

### Claim 6 (depends on 5)

The method according to claim 5, wherein the aqueous starch solution is treated:
with 40 000 to 150 000 U of branching enzyme per 100 g of starch, at a temperature of between 25 and 80° C. for a period of 7 to 24 hours,
and then with 0.05 to 0.5% ml of β-amylase per 100 g of starch, at a temperature of 60° C., a pH of 4.9 to 5, for a period of between 1 and 3 hours.

### Claim 7 (depends on 5)

The method according to claim 5, wherein the aqueous starch solution has between at least 40% and at most 60% by weight of amylose.

### Claim 8 (depends on 5)

The method according to claim 5, wherein the aqueous starch solution has between at least 60% and at most 80% by weight of standard amylose.

### Claim 9 (depends on 1)

A composition comprising the soluble highly branched glucose polymers according to claim 1.

### Claim 10 (depends on 2)

The polymers according to claim 2, have between at least 12% and at most 14% with a DP of between 15 and 25.

### Claim 11 (depends on 2)

The polymers according to claim 2, have between at least 10% and at most 12% with a DP of between 15 and 25.

### Claim 12 (depends on 2)

A method for preparing the soluble highly branched glucose polymers according to claim 2, comprising:
i) preparing an aqueous starch solution having an amylose content of at least 30% by weight,
ii) treating the said solution with a branching enzyme and then a β-amylase successively,
iii) carrying out a fractionation so as to recover the high molecular weight fractions, and
iv) collecting the highly branched glucose polymers thus obtained.

### Claim 13 (depends on 3)

A method for preparing the soluble highly branched glucose polymers according to claim 3, comprising:
i) preparing an aqueous starch solution having an amylose content of at least 30% by weight,
ii) treating the said solution with a branching enzyme and then a β-amylase successively,
iii) carrying out a fractionation so as to recover the high molecular weight fractions, and
iv) collecting the highly branched glucose polymers thus obtained.

### Claim 14 (depends on 4)

A method for preparing the soluble highly branched glucose polymers according to claim 4, comprising:
i) preparing an aqueous starch solution having an amylose content of at least 30% by weight,
ii) treating the said solution with a branching enzyme and then a β-amylase successively,
iii) carrying out a fractionation so as to recover the high molecular weight fractions, and
iv) collecting the highly branched glucose polymers thus obtained.

### Claim 15 (depends on 5)

The method according to claim 5, wherein the amylase contents is between 35 and 80° C.

### Claim 16 (depends on 6)

The method according to claim 6, wherein the aqueous starch solution is treated for a period of between 18 and 24 hours, and with α-amylase for 2 hours.

### Claim 17 (depends on 12)

The method according to claim 12, wherein the amylase contents is between 35 and 80° C.

### Claim 18 (depends on 13)

The method according to claim 13, wherein the amylase contents is between 35 and 80° C.

### Claim 19 (depends on 14)

The method according to claim 14, wherein the amylase contents is between 35 and 80° C.

### Claim 20 (depends on 9)

The composition according to claim 9, wherein said composition is in the form of a food.

### Claim 21 (depends on 9)

The composition according to claim 9, wherein said composition is in the form of a medicinal product.