# US 7,612,177 B2 · Protein for use in modifying abiotic stress tolerance in yeast

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- Number: US 7,612,177 B2
- Document ID: US7612177B2
- Status: Granted
- Kind: B2
- Application number: 10/552,686
- Filed: 2004-04-13
- Published: 2006-09-07
- Granted: 2009-11-03
- Earliest claimed: 2003-04-11
- Assignees: CropDesign N.V.
- Inventors: Jose Miguel Mulet Salort; Ramon Serrano Salom
- Web page: https://patentgrep.com/patent/US7612177B2

## CPC

- C12N 15/8273: {for drought, cold, salt resistance}
- C07K 14/415: from plants
- C12N 15/81: for yeasts
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

A method is presented for selecting and isolating nucleic acids capable of conferring tolerance or resistance to environmental stress conditions in plants or yeast. Furthermore, nucleic acids, the proteins they encode and their use for the production of plants or yeast with enhanced environmental stress resistance is disclosed.

## Claims

### Claim 1

An isolated protein comprising the sequence shown in SEQ ID NO: 4.

### Claim 2

An isolated protein consisting of the sequence shown in SEQ ID NO: 4.

### Claim 3

An isolated variant of a protein comprising the amino acid shown in SEQ ID NO:4, wherein the variant comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:4, said variant being capable of increasing stress tolerance of a yeast cell.

### Claim 4 (depends on 3)

The isolated variant protein of claim 3 wherein the variant comprises an amino acid sequence that is at least 96% identical to SEQ ID NO:4.

### Claim 5 (depends on 3)

The isolated variant protein of claim 3 wherein the variant comprises an amino acid sequence that is at least 97% identical to SEQ ID NO:4.

### Claim 6 (depends on 3)

The isolated variant protein of claim 3 wherein the variant comprises an amino acid sequence that is at least 98% identical to SEQ ID NO:4.

### Claim 7 (depends on 3)

The isolated variant protein of claim 3 wherein the variant comprises an amino acid sequence that is at least 99% identical to SEQ ID NO:4.

### Claim 8 (depends on 1)

A method of increasing abiotic stress tolerance in yeast comprising transforming yeast with a nucleic acid sequence encoding a protein of claim 1, and expressing the protein of claim 1 in said yeast such that said yeast demonstrate increased abiotic stress tolerance as compared with control yeast.

### Claim 9 (depends on 1, 2)

A method of increasing abiotic stress tolerance in yeast comprising transforming yeast with a nucleic acid sequence encoding a protein of claim 1, and expressing the protein of claim 2 in said yeast such that said yeast demonstrate increased abiotic stress tolerance as compared with control yeast.

### Claim 10 (depends on 8)

The method of claim 8 wherein said abiotic stress is temperature stress.

### Claim 11 (depends on 9)

The method of claim 9 wherein said abiotic stress is temperature stress.

### Claim 12 (depends on 8)

The method of claim 8 wherein said abiotic stress is cold temperature stress.

### Claim 13 (depends on 9)

The method of claim 9 wherein said abiotic stress is cold temperature stress.