# US 7,611,889 B2 · Method for noncovalently immobilizing a biomolecule on a solid substrate and microarray produced according to the method

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- Number: US 7,611,889 B2
- Document ID: US7611889B2
- Status: Granted
- Kind: B2
- Application number: 11/052,345
- Filed: 2005-02-07
- Published: 2005-08-18
- Granted: 2009-11-03
- Earliest claimed: 2004-02-16
- Assignees: Samsung Electronics Co., Ltd.
- Inventors: Nam Huh; Jong-myeon Park
- Web page: https://patentgrep.com/patent/US7611889B2

## CPC

- C12Q 1/6837: using probe arrays or probe chips (C12Q1/6874 takes precedence)
- G01N 33/54393: {Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding}
- B01J 2219/00387: Applications using probes
- B01J 2219/00529: DNA chips
- B01J 2219/00596: Solid-phase processes
- B01J 2219/00608: DNA chips
- B01J 2219/00612: the surface being inorganic
- B01J 2219/0063: Other, e.g. van der Waals forces, hydrogen bonding
- B01J 2219/00637: by coating it with another layer
- B01J 2219/00659: Two-dimensional arrays
- B01J 2219/00677: Ex-situ synthesis followed by deposition on the substrate
- C40B 60/14: for creating libraries
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- C40B: COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES (in silico combinatorial libraries of nucleic acids, proteins or peptides G16B35/00; in silico combinatorial chemistry G16C20/60)

## Abstract

Provided is a method for noncovalently immobilizing a biomolecule on a solid substrate, including: providing a solid substrate having a first functional group attached thereto, the first functional group having a hydrogen bond donating ability; and reacting a mixture of a compound having a hydrogen bond accepting ability and a biomolecule functionalized with a second functional group, with the surface of the substrate, the second functional group having a hydrogen bond donating ability, in order to noncovalently immobilize the biomolecule on the substrate.

## Claims

### Claim 1

A method of producing a microarray, comprising:
providing a nonporous solid substrate having an amino functional group attached thereto;
dissolving a nucleic acid functionalized with an amino group in a solution comprising polyethylene glycol (PEG) to form a solution containing the nucleic acid and the PEGS; and
transferring the solution containing the nucleic acid and the PEG to the substrate, in order to noncovalently immobilize the nucleic acid on the substrate via noncovalent intervention of the PEG;
wherein the nonporous solid substrate having an amino functional group attached thereto is a solid substrate with a surface coated with γ-aminopropyltriethoxysilane (GAPS), and
the nucleic acid functionalized with an amino group is DNA functionalized with an aminohexyl group at its 5′ end, and
wherein a concentration of the PEG is in a range of 0.4 to 8.0 mM.

### Claim 2 (depends on 1)

The method of claim 1, wherein the PEG has a molecular weight of 200 to 1,000,000 Da.

### Claim 3 (depends on 1)

The method of claim 1, wherein a concentration of the nucleic acid is in a range of 20 to 100 μM.

### Claim 4 (depends on 1)

A microarray produced according to the method of claim 1.