# US 7,611,882 B2 · Detergent compositions comprising Bacillus subtilis pectate lyases

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- Number: US 7,611,882 B2
- Document ID: US7611882B2
- Status: Granted
- Kind: B2
- Application number: 10/478,840
- Filed: 2002-05-14
- Published: 2006-07-27
- Granted: 2009-11-03
- Earliest claimed: 2001-05-14
- Assignees: Novozymes A/S
- Applicants: Torben Henriksen, legal representative
- Inventors: Mads Eskelund Bjørnvad; Martin Schülein; Vibeke Skovgaard Nielsen; Johan Smets; Carsten Andersen; Thomas Thisted
- Web page: https://patentgrep.com/patent/US7611882B2

## CPC

- A61K 8/66: Enzymes
- A61Q 11/00: Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
- C11D 3/38636: {containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase}
- C12N 9/88: Lyases (4.)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Detergent composition comprising a surfactant and a pectate lyase (EC 4.2.2.2) enzyme en-coded by a DNA sequence endogeneous to a strain of Bacillus subtilis or a stabilized variant thereof provides superior cleaning and stain removal.

## Claims

### Claim 1

An isolated pectate lyase comprising the sequence of amino acids 1-399 of SEQ ID NO: 2.

### Claim 2 (depends on 1)

The pectate lyase of claim 1, consisting of the sequence of amino acids 1-399 of SEQ ID NO: 2.

### Claim 3

A detergent composition comprising
(a) a surfactant and
(b) a pectate lyase comprising the sequence of amino acids 1-399 of SEQ ID NO: 2.

### Claim 4 (depends on 3)

The detergent composition of claim 3, wherein the pectate lyase consists of the sequence of amino acids 1-399 of SEQ ID NO: 2.

### Claim 5 (depends on 3)

The detergent composition of claim 3, wherein the pectate lyase is present at a level of from 0.0001% to 2% pure enzyme by weight of total composition.

### Claim 6 (depends on 3)

The detergent composition of claim 3, which further comprises one or more enzymes selected from the group consisting of proteases, cellulases (endoglucanases), beta-glucanases, hemicellulases, lipases, peroxidases, laccases, alpha-amylases, glucoamylases, cutinases, pectinases, reductases, oxidases, phenoloxidases, ligninases, pullulanases, pectate lyases, xyloglucanases, xylanases, pectin acetyl esterases, polygalacturonases, rhamnogalacturonases, pectin lyases, mannanases, pectin methylesterases, cellobiohydrolases, transglutaminases; and mixtures thereof.

### Claim 7 (depends on 3)

The detergent composition of claim 3, wherein the surfactant is an anionic surfactant and is present at a level up to 30% by weight.

### Claim 8 (depends on 3)

The detergent composition of claim 3, which further comprises a bleaching agent.

### Claim 9 (depends on 3)

A method of cleaning a fabric, dishware or hard surface, comprising treating the fabric, dishware or hard surface with a detergent composition of claim 3.