# US 7,611,859 B2 · Method for improving heat stability of composition containing water-soluble coenzyme-bound glucose dehydrogenase (GDH)

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- Number: US 7,611,859 B2
- Document ID: US7611859B2
- Status: Granted
- Kind: B2
- Application number: 11/392,000
- Filed: 2006-03-29
- Published: 2007-05-10
- Granted: 2009-11-03
- Earliest claimed: 2005-11-10
- Assignees: Toyo Boseki Kabushiki Kaisha
- Inventors: Masao Kitabayashi
- Web page: https://patentgrep.com/patent/US7611859B2

## CPC

- A61B 5/14532: {for measuring glucose, e.g. by tissue impedance measurement}
- A61B 5/1486: using enzyme electrodes, e.g. with immobilised oxidase
- C12N 9/0006: {acting on CH-OH groups as donors (1.1)}
- C12N 9/96: Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
- C12Q 1/006: {for glucose}
- C12Q 1/32: involving dehydrogenase
- C12Q 1/54: involving glucose or galactose
- A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The invention relates to a method for improving the heat stability of glucose dehydrogenase (GDH), comprising a step of combining, in a composition comprising soluble coenzyme-bound glucose dehydrogenase (GDH), the enzyme with any one or more selected from the group consisting of sugar alcohols, carboxyl group-containing compounds, alkali metal-containing compounds, alkali earth metal compounds, ammonium salts, sulfate salts and proteins, thereby improving the heat stability of GDH over that achieved without the inclusion of the compounds.

## Claims

### Claim 1

A method for preparing a glucose sensor comprising (a) combining bovine serum albumin in a composition comprising soluble flavin-bound glucose dehydrogenase (GDH), thereby improving the heat stability of GDH over that achieved without the inclusion of the bovine serum albumin, and (b) heat drying the composition onto the surface of the glucose sensor.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the concentration of bovine serum albumin is 0.1% by weight or more after combining bovine serum albumin with the composition comprising soluble flavin-bound glucose dehydrogenase and before heat drying the composition.

### Claim 3 (depends on 1)

A glucose sensor prepared by the method of claim 1.

### Claim 4 (depends on 3)

A method of measuring glucose concentration using the glucose sensor of claim 3.

### Claim 5 (depends on 2)

A glucose sensor prepared by the method of claim 2.

### Claim 6 (depends on 5)

A method of measuring glucose concentration using the glucose sensor of claim 5.