# US 7,611,851 B2 · Canis sphingosine 1-phosphate receptor isoform 1

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- Number: US 7,611,851 B2
- Document ID: US7611851B2
- Status: Granted
- Kind: B2
- Application number: 11/658,345
- Filed: 2005-07-22
- Published: 2009-07-09
- Granted: 2009-11-03
- Earliest claimed: 2004-07-27
- Assignees: Merck & Co., Inc.
- Inventors: Suzanne M. Mandala; Cheryl Meyers; Gan-Ju Maria Shei
- Web page: https://patentgrep.com/patent/US7611851B2

## CPC

- C07K 14/705: Receptors; Cell surface antigens; Cell surface determinants {(tumour specific antigens C07K14/4748)}
- C07K 14/4722: {G-proteins}
- C07K 14/723: {G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor}
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

A Canis sphingosine-1-phosphate (S1P) receptor isoform 1 (cS1P1), the nucleic acid encoding the cS1P1 receptor, and methods for using the cS1P1 receptor and the nucleic acid encoding the cS1P1 receptor in assays for identifying analytes which modulate activity of the cS1P1 receptor.

## Claims

### Claim 1

An isolated nucleic acid molecule comprising a nucleotide sequence encoding a Canis sphingosine-1-phosphate isoform 1 (cS1P1) receptor which comprises the amino acid sequence set forth in SEQ ID NO:2.

### Claim 2

An isolated nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:1.

### Claim 3

An isolated protein comprising the amino acid sequence Of SEQ ID NO:2.

### Claim 4

A method for identifying an analyte that modulates activity of a Canis sphingosine-1-phosphate receptor isoform 1 (cS1P1) receptor, which comprises:
(a) providing a recombinant cell comprising an isolated nucleic acid molecule which encodes a cS1P1 receptor comprising the amino acid sequence set forth in SEQ ID NO: 2;
(b) incubating the recombinant cell in a medium with the analyte such that the cell expresses the cS1P1 receptor; and
(c) determining the activity of the cS1P1 receptor wherein a change in the activity of the cS1P1 receptor indicates the analyte modulates activity of the cS1P1 receptor.

### Claim 5 (depends on 4)

The method of claim 4 wherein the activity of the cS1P1 is determined by measuring a change in the intracellular concentration of Ca2+ in the presence of the analyte.

### Claim 6 (depends on 4)

The method of claim 4 wherein the activity of the cS1P1 is determined by measuring a change in the intracellular concentration of a metabolite selected from the group consisting of inositol triphosphate (IP3) and diacylglycerol (DAG) in the presence of the analyte.

### Claim 7 (depends on 4)

The method of claim 4 wherein the activity of the cS1P1 is determined by measuring a change in the activity of phospholipase C beta (PLCβ) or protein kinase C (PKC) in the presence of the analyte.

### Claim 8 (depends on 4)

The method of claim 4 wherein the activity of the cS1P1 is determined by measuring a change in the synthesis of cyclic AMP (cAMP) in the presence of the analyte.

### Claim 9 (depends on 4)

The method of claim 4 wherein the isolated nucleic acid molecule which encodes the cS1P1 receptor comprises the nucleotide sequence set forth in SEQ ID NO:1.