# US 7,611,836 B2 · Method of making a plastic colorimetric resonant biosensor device with liquid handling capabilities

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- Number: US 7,611,836 B2
- Document ID: US7611836B2
- Status: Granted
- Kind: B2
- Application number: 11/673,626
- Filed: 2007-02-12
- Published: 2007-06-21
- Granted: 2009-11-03
- Earliest claimed: 2000-10-30
- Assignees: SRU Biosystems, Inc.
- Inventors: Jean Qiu; Peter Li; Brian T. Cunningham
- Web page: https://patentgrep.com/patent/US7611836B2

## CPC

- G01N 21/253: {for batch operation, i.e. multisample apparatus (analytical automats G01N35/00)}
- B01L 3/5085: {for multiple samples, e.g. microtitration plates}
- G01N 21/4788: {Diffraction (for sizing particles G01N15/0205)}
- G01N 21/7743: {the reagent-coated grating coupling light in or out of the waveguide}
- G01N 33/54373: {involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings}
- G02B 5/1809: {with pitch less than or comparable to the wavelength}
- G02B 21/34: Microscope slides, e.g. mounting specimens on microscope slides
- B01L 2200/0689: Sealing
- B01L 2300/0636: Integrated biosensor, microarrays
- B01L 2300/0819: Microarrays; Biochips
- B01L 2300/0829: Multi-well plates; Microtitration plates
- Y10T 156/10: Methods of surface bonding and/or assembly therefor
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

Methods of producing liquid handling biosensor devices are provided. The liquid handling biosensor devices allow detection of biomolecular interactions in liquid. The use of labels is not required and the methods can be performed in a high-throughput manner.

## Claims

### Claim 1

A method of making a liquid handling colorimetric resonant reflection biosensor device, comprising:
a. applying an attachment material to a surface of a transfer block,
b. contacting a liquid holding part with the attachment material on the surface of the transfer block,
c. removing the liquid holding part and any attachment material present on the liquid holding part from the transfer block,
d. contacting a biosensor with the liquid holding part so that the attachment material is between the biosensor and the liquid holding part, and
e. exposing the liquid holding part and biosensor to ultraviolet light to solidify the attachment material, wherein the liquid holding part is immobilized onto the biosensor.

### Claim 2 (depends on 1)

The method of claim 1, wherein the biosensor comprises a grating comprised of or coated with a dielectric material having a high refractive index or a reflective material, wherein when the biosensor is illuminated a resonant grating effect is produced on the reflected radiation spectrum.

### Claim 3 (depends on 1)

The method of claim 1, wherein the attachment material is an ultraviolet (UV) curable polymer.

### Claim 4 (depends on 1)

The method of claim 1, wherein the liquid holding part is a bottomless microtiter plate or a fluid flow channel.

### Claim 5 (depends on 1)

The method of claim 1, wherein the attachment material is applied by a knife coating technique or a rod coating technique.

### Claim 6 (depends on 1)

The method of claim 1, wherein the step of contacting further comprises placing the biosensor in a first part of a holding fixture and placing the liquid handling part in a second part of the holding fixture, wherein the first and second parts of the holding fixture are brought together so that the biosensor and the liquid holding part are in contact with each other.

### Claim 7 (depends on 1)

The method of claim 1, wherein the thickness of the attachment material on the surface of the transfer block is about 0.025 mm to about 5 mm thick.