# US 7,611,830 B2 · Device to lavage a blood vessel > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 7,611,830 B2 - Document ID: US7611830B2 - Status: Granted - Kind: B2 - Application number: 10/257,176 - Filed: 2001-04-10 - Published: 2004-05-27 - Granted: 2009-11-03 - Earliest claimed: 2000-04-10 - Assignees: The United States of America as represented by the Department of Veterans Affairs - Inventors: Hemant Thatte; Kunda Biswas; Shukri Khuri; Thomas Michel - Web page: https://patentgrep.com/patent/US7611830B2 ## CPC - A01N 1/10: Preservation of living parts - A01N 1/126: Physiologically active agents, e.g. antioxidants or nutrients - A01N 1/143: for organ perfusion - A61K 9/0009: {involving or responsive to electricity, magnetism or acoustic waves; Galenical aspects of sonophoresis, iontophoresis, electroporation or electroosmosis} - A61K 31/198: Alpha-amino acids, e.g. alanine or edetic acid [EDTA] (betaine A61K31/205; proline A61K31/401; tryptophan A61K31/405; histidine A61K31/4172; peptides not degraded to individual amino acids A61K38/00) - A61K 31/375: Ascorbic acid, i.e. vitamin C; Salts thereof - A61K 31/70: Carbohydrates; Sugars; Derivatives thereof (sorbitol A61K31/047) - A61K 31/727: Heparin; Heparan - A61K 35/44: Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells - A61K 38/063: {Glutathione} - A61K 38/58: from leeches, e.g. hirudin, eglin - Y10S 623/916: Blood vessel - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS ## Abstract The present invention provides for compositions and methods for the preservation of tissues and organs ex vivo and in situ. In addition, the present invention provides for kits that may be used in the preparation of the solutions of the present invention. The present invention also provides a device for perfusing tissues and organs with the solutions of the present invention. ## Claims ### Claim 1 A device comprising, an incomplete, hollow circuit defining a liquid flow path in fluidic communication with a chamber comprising an inlet port and an outlet port, said incomplete circuit comprising a pump operably linked to a pressure transducer, said circuit terminating at first and second attachment points, wherein said circuit comprises an efflux port disposed between said inlet port and said first attachment point and an uptake port disposed between said outlet port and said second attachment point, wherein the bore of said uptake port bore is larger than the bore of said efflux port thereby resulting in a bore size differential creating enough turbulence to continuously lavage a blood vessel, such that attachment of a first end of said blood vessel to said first attachment point and a second end of said blood vessel to said second attachment point generates a complete, hollow circuit defining a liquid flow path. ### Claim 2 (depends on 1) The device of claim 1, wherein said first attachment point comprises a serrated nozzle. ### Claim 3 (depends on 1) The device of claim 1, wherein second attachment point comprises an adjustable serrated nozzle. ### Claim 4 (depends on 1) The device of claim 1, wherein said chamber is sterile. ### Claim 5 (depends on 1) The device of claim 1, wherein said chamber is disposable. ### Claim 6 (depends on 1) The device of claim 1, wherein said incomplete, hollow circuit comprises tubing, said tubing terminating inside said chamber at said first and second attachment points. ### Claim 7 (depends on 1) The device of claim 1, wherein said tubing is in fluidic communication with a reservoir. ### Claim 8 A method for perfusion, comprising: a) providing i) a device comprising, an incomplete, hollow circuit defining a liquid flow path in fluidic communication with a chamber comprising an inlet port and an outlet port, said incomplete circuit comprising a pump operably linked to a pressure transducer, said circuit terminating at first and second attachment points, wherein said circuit comprises an efflux port disposed between said inlet port and said first attachment point and an uptake port disposed between said outlet port and said second attachment point, wherein the bore of said uptake port bore is larger than the bore of said efflux port thereby resulting in a bore size differential creating enough turbulence to continuously lavage a blood vessel; and ii) a segment of a blood vessel, said blood vessel having a first end and a second end; b) attaching, in any order, said first end of said segment to said first attachment point and said second end of said segment to said second attachment point, under conditions such that a complete, hollow circuit defining a liquid flow path is produced; and c) circulating an aqueous solution in said hollow circuit. ### Claim 9 (depends on 8) The method of claim 8, wherein said aqueous solution comprises an antioxidant and L-arginine. ### Claim 10 (depends on 9) The method of claim 9, wherein said aqueous solution further comprises an anticoagulant. ### Claim 11 (depends on 10) The method of claim 10, further comprising a cellular reducing agent. ### Claim 12 (depends on 8) The method of claim 8, wherein said blood vessel is an isolated vein. ### Claim 13 (depends on 12) The method of claim 12, wherein said vein is a saphenous vein.