# US 7,608,757 B2 · Signal transduction stress-related proteins and methods of use in plants > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 7,608,757 B2 - Document ID: US7608757B2 - Status: Granted - Kind: B2 - Application number: 11/566,246 - Filed: 2006-12-04 - Published: 2007-07-05 - Granted: 2009-10-27 - Earliest claimed: 2000-04-07 - Assignees: BASF Plant Science GmbH - Inventors: Oswaldo da Costa e Silva; Hans J. Bohnert; Nocha van Thielen; Ruoying Chen; Manabu Ishitani - Web page: https://patentgrep.com/patent/US7608757B2 ## CPC - C12N 15/8273: {for drought, cold, salt resistance} - C07K 14/415: from plants - C12N 9/1205: {Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases} - C12N 9/16: acting on ester bonds (3.1) - C12N 15/8217: {Gene switch} - A01K 2217/05: Animals comprising random inserted nucleic acids (transgenic) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS ## Abstract A transgenic plant transformed by a Signal Transduction Stress-Related Protein (STSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in increased tolerance to environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated STSRPs, and isolated nucleic acid coding STSRPs, and vectors and host cells containing the latter. ## Claims ### Claim 1 A transgenic plant cell transformed with an expression cassette comprising an isolated polynucleotide encoding a full-length 14-3-3 polypeptide having a sequence comprising amino acids 1 to 258 of SEQ ID NO:13. ### Claim 2 (depends on 1) The plant cell of claim 1, wherein the polynucleotide has a sequence comprising nucleotides 1 to 916 of SEQ ID NO:8. ### Claim 3 A transgenic plant transformed with an expression cassette comprising an isolated polynucleotide encoding a full-length 14-3-3 polypeptide having a sequence comprising amino acids 1 to 258 of SEQ ID NO:13. ### Claim 4 (depends on 3) The plant of claim 3, wherein the polynucleotide has a sequence comprising nucleotides 1 to 916 of SEQ ID NO:8. ### Claim 5 (depends on 3) The plant of claim 3, further described as a monocot. ### Claim 6 (depends on 3) The plant of claim 3, further described as a dicot. ### Claim 7 (depends on 3) The plant of claim 3, wherein the plant is selected from the group consisting of maize, wheat, rye, oat, triticale, rice, barley, soybean, peanut, cotton, rapeseed, canola, manihot, pepper, sunflower, tagetes, potato, tobacco, eggplant, tomato, Vicia species, pea, alfalfa, coffee, cacao, tea, Salix species, oil palm, coconut, perennial grasses, and a forage crop plant. ### Claim 8 A seed which is true breeding for a transgene comprising a polynucleotide encoding a full-length 14-3-3 polypeptide having a sequence comprising amino acids 1 to 258 of SEQ ID NO:13. ### Claim 9 (depends on 8) The seed of claim 8, wherein the polynucleotide has a sequence comprising nucleotides 1 to 916 of SEQ ID NO:8. ### Claim 10 An isolated polynucleotide having a sequence comprising nucleotides 1 to 916 of SEQ ID NO:8. ### Claim 11 An isolated polynucleotide encoding a polypeptide having a sequence comprising amino acids 1 to 258 of SEQ ID NO:13. ### Claim 12 A method of producing a drought-tolerant transgenic plant, the method comprising the steps of: a) transforming a plant cell with an expression cassette comprising a polynucleotide encoding a full-length 14-3-3 polypeptide having a sequence comprising amino acids 1 to 258 of SEQ ID NO:13; b) growing the transformed plant cell to generate transgenic plants; and c) screening the transgenic plants generated in step b) to identify a transgenic plant that expresses the polypeptide and exhibits increased tolerance to drought stress as compared to a wild type variety of the plant. ### Claim 13 (depends on 12) The method of claim 12, wherein the polynucleotide has a sequence comprising nucleotides 1 to 916 of SEQ ID NO:8. ### Claim 14 (depends on 7) The plant of claim 7, which is maize. ### Claim 15 (depends on 7) The plant of claim 7, which is rice. ### Claim 16 (depends on 7) The plant of claim 7, which is soybean. ### Claim 17 (depends on 7) The plant of claim 7, which is cotton. ### Claim 18 (depends on 7) The plant of claim 7, which is rapeseed or canola.