# US 7,608,756 B2 · Promoters for epidermis-specific expression of desired coding sequences in transgenic plants

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- Number: US 7,608,756 B2
- Document ID: US7608756B2
- Status: Granted
- Kind: B2
- Application number: 10/574,740
- Filed: 2004-10-07
- Published: 2007-12-27
- Granted: 2009-10-27
- Earliest claimed: 2003-10-07
- Assignees: IPK-Institut Fur Pflanzengenetik Und Kulturpflanzenforschung; Max-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.; UniversitäT ZüRich
- Inventors: Patrick Schweizer; Robert Dudler; Paul Schulze-Lefert; Ralph Panstruga
- Web page: https://patentgrep.com/patent/US7608756B2

## CPC

- C12N 15/8222: {Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation}
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The invention relates to methods of producing transgenic plants including pathogen resistant plants with recombinant nucleic acid molecules comprising promoter regions which provides expression of a desired transgene in epidermis-specific manner, and transgenic plants produced by said methods.

## Claims

### Claim 1

A method of causing epidermis specific expression of a desired coding sequence in a transgenic plant, the method comprising introducing into a plant cell a chimeric gene comprising an isolated promoter region that controls epidermis-specific expression operably linked to the desired coding sequence, said promoter region comprising a first sequence originating from the promoter of a GSTA1 gene and a second sequence originating from the intron of a WIR1a gene, wherein the first sequence is SEQ ID No. 1 and the second sequence is SEQ ID No. 2 or wherein the first sequence has at least 95% sequence identity to SEQ ID No. 1 and the second sequence has at least 95% sequence identity to SEQ ID No. 2.

### Claim 2

A method for increasing pathogen resistance in a plant, the method comprising transforming a plant cell with a chimeric gene comprising an isolated promoter region that controls epidermis-specific expression operably linked to a DNA encoding a protein that confers pathogen resistance, said promoter region comprising a first sequence originating from the promoter of a GSTA1 gene and a second sequence originating from the intron of a WIR1a gene, wherein the first sequence is SEQ ID No. 1 and the second sequence is SEQ ID No. 2 or wherein the first sequence has at least 95% sequence identity to SEQ ID No. 1 and the second sequence has at least 95% sequence identity to SEQ ID No. 2; and regenerating a transformed plant from the transformed plant cell; said transformed plant exhibits increased resistance to a pathogen.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the first sequence is SEQ ID No. 1 and the second sequence is SEQ ID No. 2.

### Claim 4 (depends on 2)

The method according to claim 2, wherein the first sequence is SEQ ID No. 1 and the second sequence is SEQ ID No. 2.

### Claim 5 (depends on 1)

The method according to claim 1, wherein said isolated promoter region comprises the nucleic acid sequence of SEQ ID NO. 3, or has at least 95% sequence identity to the nucleic acid sequence of SEQ ID No. 3.

### Claim 6 (depends on 2)

The method according to claim 2, wherein said isolated promoter region comprises the nucleic acid sequence of SEQ ID NO. 3, or has at least 95% sequence identity to the nucleic acid sequence of SEQ ID No. 3.

### Claim 7 (depends on 1)

The method according to claim 1, wherein the desired coding sequence originates from a resistance gene.

### Claim 8 (depends on 7)

The method according to claim 7, wherein the coding sequence encodes a peroxidase or an oxalate oxidase.

### Claim 9 (depends on 1)

The method of claim 1, wherein the coding sequence is in antisense orientation.

### Claim 10 (depends on 1)

A transgenic plant produced by the method of claim 1.

### Claim 11 (depends on 2)

A transgenic plant produced by the method of claim 2.

### Claim 12 (depends on 10)

The transgenic plant of claim 10, wherein said plant is a monocot or dicot plant.

### Claim 13 (depends on 11)

The transgenic plant of claim 11, wherein said plant is a monocot or dicot plant.

### Claim 14 (depends on 12)

The transgenic plant according to claim 12, wherein said plant is poaceae.

### Claim 15 (depends on 13)

The transgenic plant according to claim 13, wherein said plant is poaceae.

### Claim 16 (depends on 14)

The transgenic plant according to claim 14, wherein said plant is wheat or barley.

### Claim 17 (depends on 15)

The transgenic plant according to claim 15, wherein said plant is wheat or barley.