# US 7,608,751 B2 · Fungus resistant plants and their uses

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- Number: US 7,608,751 B2
- Document ID: US7608751B2
- Status: Granted
- Kind: B2
- Application number: 10/567,980
- Filed: 2004-08-03
- Published: 2006-11-02
- Granted: 2009-10-27
- Earliest claimed: 2003-08-11
- Assignees: Kweek-En Researchbedruf Agrico B.V.
- Inventors: Edwin Andries Gerard Van Der Vossen; Josephus Jacoubs Henricus Maria Allefs; Marina Woutera Maria Muskens
- Web page: https://patentgrep.com/patent/US7608751B2

## CPC

- C07K 14/415: from plants
- C12N 15/8282: {for fungal resistance}
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to a novel method for increasing the resistance, of a plant, in particular of a Solanaceae, preferably of potato and tomato, to plant pathogens of the phylum Oomyceta comprising increasing the activity of the polypeptid of the present invention. The invention further relates to polynucleotides and vectors comprising these polynucleotides. The invention furthermore relates to corresponding vectors, cells, transgenic plants and transgenic propagation material derived from them, methods to produce them and to their use for the production of foodstuffs, feeding stuffs, seed, pharmaceuticals or fine chemicals.

## Claims

### Claim 1

A method for generating or increasing the resistance of a plant to a plant pathogen of the phylum Oomyceta comprising increasing the activity of a Rpi-blb2 protein in the plant or a tissue, organ or cell of the plant or a part thereof by expressing a transgenic Rpi-blb2 protein encoding nucleic acid molecule, wherein the plant has increased resistance to the plant pathogen of the phylum Oomyceta, wherein said Rpi-blb2 protein encoding nucleic acid molecule is selected from the group consisting of:
(a) a nucleic acid molecule encoding the polypeptide depicted in SEQ ID NO: 2 or 4;
(b) a nucleic acid molecule comprising the coding sequence as depicted in SEQ ID NO: 3 or 5 or 6; and
(c) a nucleic acid molecule encoding a polypeptide comprising a sequence having at least 95% identity to the amino acid sequence of the polypeptide encoded by the nucleic acid molecule of (a) or (b).

### Claim 2 (depends on 1)

The method of claim 1 which results in reduction in sporulation index of at least 30% after infection with P. infestans compared to a wild type.

### Claim 3 (depends on 1)

The method of claim 1, wherein the Rpi-blb2 protein comprises by a P-loop and a NBS domain.

### Claim 4 (depends on 1)

The method of claim 1, wherein the Rpi-blb2 protein encoding nucleic acid molecule is a nucleic acid molecule encoding a polypeptide comprising a sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 2 or 4, wherein the polypeptide comprises a NBS domain and an LRR domain.