# US 7,608,699 B2 · Synthetic nuclear localization signal derived from lentiviral integrase and methods of use thereof

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- Number: US 7,608,699 B2
- Document ID: US7608699B2
- Status: Granted
- Kind: B2
- Application number: 11/023,996
- Filed: 2004-12-29
- Published: 2006-06-22
- Granted: 2009-10-27
- Earliest claimed: 2003-12-29
- Assignees: The Rockefeller University
- Inventors: Mark A. Muesing; Tshaka J. Cunningham
- Web page: https://patentgrep.com/patent/US7608699B2

## CPC

- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- C07K 14/005: from viruses
- C07K 2319/09: containing a nuclear localisation signal
- C12N 2740/16061: Methods of inactivation or attenuation
- C12N 2740/16222: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The present invention provides novel peptides, nucleic acids, vectors, compounds, compositions and methods for regulating nuclear import. The present invention also relates to a lentiviral NLS, and methods of use thereof for inhibiting HIV pathogenesis and disease progression, and for gene delivery methods.

## Claims

### Claim 1

An isolated polynucleotide comprising a nucleic acid encoding a lentiviral nuclear localization signal derived from a fragment of an HIV-1 integrase consisting of the amino acid sequence corresponding to the formula:
R5(R6)2FRVYR1R3R2R8R3NPLWR3GPR8KR4R5WR3GEGA(R4)3R6DR6SR6R4KVR4PR(R3)2R8KIR4R3D, wherein:
R1=an aromatic amino acid;
R2=an acidic amino acid;
R3=a basic amino acid;
R4=a branched chain amino acid;
R5=a hydrophobic amino acid;
R6=a polar amino acid;
R7=a proline or serine amino acid residue;
R8=a small amino acid residue selected from the group consisting of a glycine, an alanine, a cysteine and an asparagine amino acid residue.