# US 7,608,453 B2 · Intracellular antibodies

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- Number: US 7,608,453 B2
- Document ID: US7608453B2
- Status: Granted
- Kind: B2
- Application number: 10/771,257
- Filed: 2004-02-03
- Published: 2005-12-29
- Granted: 2009-10-27
- Earliest claimed: 2002-08-01
- Assignees: MRC Sissa-Scuola Internazionale Superiore de Studi Avanzati
- Inventors: Antonino Cattaneo; Amos Maritan; Michela Visintin; Terrence Rabbitts; Giovanni Settanni
- Web page: https://patentgrep.com/patent/US7608453B2

## CPC

- G16B 30/10: Sequence alignment; Homology search
- G16B 30/00: ICT specially adapted for sequence analysis involving nucleotides or amino acids
- G16B: BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY

## Abstract

A method of identifying at least one consensus sequence for an intracellular antibody (ICS) comprising the steps of: creating a database comprising sequences of validated intracellular antibodies (VIDA database) and aligning the sequences of validated intracellular antibodies according to Kabat; determining the frequency with which a particular amino acid occurs in each of the positions of the aligned antibodies; selecting a frequency threshold value (LP or consensus threshold) in the range from 70% to 100%; identifying the positions of the alignment at which the frequency of a particular amino acid is greater than or equal to the LP value; and identifying the most frequent amino acid, in the position of said alignment.

## Claims

### Claim 1

An intracellularly binding immunoglobulin molecule comprising: a variable heavy chain comprising the amino acid sequence of SEQ ID No 3; and a variable light chain.

### Claim 2

A method of selectively binding an intracellularly binding immunoglobulin molecule to a ligand in an intracellular environment, the method comprising contacting the intracellularly binding immunoglobulin molecule comprising a variable heavy chain comprising the amino acid sequence of SEQ ID No 3 and a variable light chain, with BCR or BCR-ABL in an intracellular environment, wherein BCR or BCR-ABL is selectively bound by said molecule.