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US 7,608,451 B2Granted
Gene regulation in transgenic animals using a transposon-based vector
AssigneesTransgen Rx, Inc., Board of Supervisors of Louisiana State University Mechanical College
InventorsRichard K. Cooper, Gary G. Cadd, William C. Fioretti, Kenneth F. De Boer
Life of this patent
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Abstract
Administration of modified transposon-based vectors has been used to achieve stable incorporation of exogenous genes into animals. These transgenic animals produce transgenic progeny. Further, these transgenic animals produce large quantities of desired molecules encoded by the transgene. Transgenic egg-laying animals produce large quantities of desired molecules encoded by the transgene and deposit these molecules in the egg.
Claims14
A vector comprising:
- a modified transposase gene operably linked to a first promoter, wherein the nucleotide sequence 3′ to the first promoter comprises a modified Kozak sequence as set forth in SEQ ID NO:
- 13, and wherein a plurality of the first twenty codons of the transposase gene are modified from the wild-type sequence by changing the nucleotide at the third base position of the codon to an adenine or thymine without modifying the amino acid encoded by the codon;
- and
- one or more genes of interest operably-linked to one or more additional promoters, wherein the one or more genes of interest and their operably-linked promoters are flanked by transposase insertion sequences recognized by a transposase encoded by the modified transposase gene
- wherein the vector comprises the sequence as set forth in SEQ ID NO:1.
2 more elements
Dependencies are inferred from the claim text.