# US 7,608,443 B2 · Lysophosphatidic acid acetyltransferases

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- Number: US 7,608,443 B2
- Document ID: US7608443B2
- Status: Granted
- Kind: B2
- Application number: 11/820,045
- Filed: 2007-06-18
- Published: 2008-01-10
- Granted: 2009-10-27
- Earliest claimed: 1999-02-22
- Assignees: E.I. du Pont de Nemours and Company
- Inventors: Anthony J. Kinney; Rebecca E. Cahoon
- Web page: https://patentgrep.com/patent/US7608443B2

## CPC

- C12N 9/1029: {transferring groups other than amino-acyl groups (2.3.1)}
- C12N 15/8247: {involving modified lipid metabolism, e.g. seed oil composition}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

An isolated nucleic acid fragment encoding an LPAAT isozyme is disclosed. Construction of a chimeric gene encoding all or a portion of the LPAAT isozyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the LPAAT isozyme in a transformed host cell is also disclosed.

## Claims

### Claim 1

An isolated polynucleotide comprising:
(a) a nucleotide sequence encoding a polypeptide having lysophosphatidic acid acyltransferase (LPAAT) activity, wherein the polypeptide has an amino acid sequence of at least 95% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO:34; or
(b) a complement of the nucleotide sequence, wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.

### Claim 2 (depends on 1)

The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide comprises SEQ ID NO:34.

### Claim 3 (depends on 1)

The polynucleotide of claim 1 wherein the nucleotide sequence comprises SEQ ID NO:33.

### Claim 4 (depends on 1)

A vector comprising the polynucleotide of claim 1.

### Claim 5 (depends on 1)

A recombinant DNA construct comprising the polynucleotide of claim 1 operable linked to at least one regulatory sequence.

### Claim 6 (depends on 1)

A method for transforming a cell, comprising transforming a cell with the polynucleotide of claim 1.

### Claim 7 (depends on 5)

A cell comprising the recombinant DNA construct of claim 5.

### Claim 8 (depends on 1)

A method for producing a plant comprising transforming a plant cell with the polynucleotide of claim 1 and regenerating a plant from the transformed plant cell.

### Claim 9 (depends on 5)

A plant comprising the recombinant DNA construct of claim 5.

### Claim 10 (depends on 5)

A seed comprising the recombinant DNA construct of claim 5.

### Claim 11 (depends on 1)

A method for isolating a polypeptide comprising isolating the polypeptide from a cell or culture medium of the cell, wherein the cell comprises a recombinant DNA construct comprising the polynucleotide of claim 1 operable linked to at least one regulatory sequence.

### Claim 12 (depends on 5)

A method of altering the level of lysophosphatidic acid acyltransferase expression in a host cell comprising:
(a) transforming a host cell with the recombinant DNA construct of claim 5; and
(b) growing the transformed host cell under conditions that are suitable for expression of the recombinant DNA construct wherein expression of the recombinant DNA construct results in production of altered levels of lysophosphatidic acid acyltransferase in the transformed host cell.