# US 7,608,442 B2 · β1,3-galactosyltransferase polypeptides from the LOS locus of C. jejuni

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- Number: US 7,608,442 B2
- Document ID: US7608442B2
- Status: Granted
- Kind: B2
- Application number: 11/548,514
- Filed: 2006-10-11
- Published: 2007-03-01
- Granted: 2009-10-27
- Earliest claimed: 1999-02-01
- Assignees: National Research Council of Canada
- Inventors: Michel Gilbert; Warren W. Wakarchuk
- Web page: https://patentgrep.com/patent/US7608442B2

## CPC

- C12N 9/1241: {Nucleotidyltransferases (2.7.7)}
- C12N 9/1029: {transferring groups other than amino-acyl groups (2.3.1)}
- C12N 9/1048: {Glycosyltransferases (2.4)}
- C12N 9/1051: {Hexosyltransferases (2.4.1)}
- C12N 9/1081: {transferring other glycosyl groups (2.4.99)}
- C12P 19/18: produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}

## Abstract

This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an α2,3- and an α2,8-activity. A β1,4-GalNAc transferase and a β1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example, Campylobacter species, including C. jejuni. In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.

## Claims

### Claim 1

An isolated or recombinant β1,3-galactosyltransferase polypeptide;
wherein a nucleotide sequence from a lipooligosaccharide (LOS) locus from Campylobacter comprises a polynucleotide sequence that encodes the β1.3-galactosyltransferase polypeptide; and
wherein the nucleotide sequence from the LOS locus is amplified by PCR using a first primer comprising SEQ ID NO:40 and a second primer comprising SEQ ID NO:41.

### Claim 2 (depends on 1)

The isolated or recombinant polypeptide of claim 1, wherein the β1,3-galactosyltransferase polypeptide further comprises a tag for purification.