# US 7,608,426 B2 · Polynucleotides encoding mammalian cytokine-like factor 7

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- Number: US 7,608,426 B2
- Document ID: US7608426B2
- Status: Granted
- Kind: B2
- Application number: 11/157,049
- Filed: 2005-06-20
- Published: 2005-12-01
- Granted: 2009-10-27
- Earliest claimed: 1997-04-25
- Assignees: ZymoGenetics, Inc.
- Inventors: Scott R. Presnell; Teresa Gilbert
- Web page: https://patentgrep.com/patent/US7608426B2

## CPC

- C07K 14/52: Cytokines; Lymphokines; Interferons
- C07K 2319/00: Fusion polypeptide
- C07K 2319/02: containing a signal sequence
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

Novel mammalian zcyto7 polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods including antibodies and anti-idiotypic antibodies.

## Claims

### Claim 1

An isolated polynucleotide that encodes a polypeptide, wherein the encoded polypeptide comprises SEQ ID NO:14.

### Claim 2 (depends on 1)

The isolated polynucleotide according to claim 1, wherein the encoded polypeptide comprises SEQ ID NO:2.

### Claim 3 (depends on 1)

The isolated polynucleotide according to claim 1, wherein the encoded polypeptide consists of SEQ ID NO:14.

### Claim 4 (depends on 1)

The isolated polynucleotide according to claim 1, wherein the encoded polypeptide consists of SEQ ID NO:2.

### Claim 5 (depends on 1)

The isolated polynucleotide according to claim 1, wherein the encoded polypeptide further comprises a second polypeptide or protein.

### Claim 6 (depends on 5)

The isolated polynucleotide according to claim 5, wherein the second polypeptide or protein is an affinity tag.

### Claim 7 (depends on 6)

The isolated polynucleotide according to claim 6, wherein the affinity tag is a poly-histidine tract, protein A, glutathione S transferase, Glu-Glu affinity tag, substance P, FLAG™ peptide, streptavidin binding peptide, immunoglobulin FC polypeptide, or other antigenic epitope or binding domain.

### Claim 8 (depends on 1)

The isolated polynucleotide according to claim 1, wherein the encoded polypeptide is fused to a carrier polypeptide or other carrier molecule.

### Claim 9

An expression vector comprising the following operably linked elements:
(a) a transcription promoter;
(b) a DNA segment that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:14; and
(c) a transcription terminator.

### Claim 10 (depends on 9)

The expression vector according to claim 9, wherein the encoded polypeptide comprises the amino acid sequence of SEQ ID NO:2.

### Claim 11 (depends on 9)

The expression vector according to claim 9, wherein the encoded polypeptide consists of the amino acid sequence of SEQ ID NO:14.

### Claim 12 (depends on 9)

The expression vector according to claim 9, wherein the encoded polypeptide consists of the amino acid sequence of SEQ ID NO:2.

### Claim 13 (depends on 9)

The expression vector according to claim 9, wherein the encoded polypeptide further comprises a second polypeptide or protein.

### Claim 14 (depends on 13)

The expression vector according to claim 13, wherein the second polypeptide or protein is an affinity tag.

### Claim 15 (depends on 14)

The expression vector according to claim 14, wherein the affinity tag is a poly-histidine tract, protein A, glutathione S transferase, Glu-Glu affinity tag, substance P, FLAG™ peptide, streptavidin binding peptide, immunoglobulin FC polypeptide, or other antigenic epitope or binding domain.

### Claim 16 (depends on 9)

A cultured cell comprising the expression vector according to claim 9, wherein the cell expresses a polypeptide encoded by the vector.

### Claim 17 (depends on 16)

A method of producing a polypeptide comprising culturing a cell according to claim 16 under conditions suitable for expression of said polypeptide, and isolating the polypeptide produced by the cell.