# US 7,608,403 B2 · Method for cancer detection, progression analysis and malign tumour prognosis based on the study of metabolic markers of the cell

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- Number: US 7,608,403 B2
- Document ID: US7608403B2
- Status: Granted
- Kind: B2
- Application number: 10/514,771
- Filed: 2003-05-20
- Published: 2007-01-04
- Granted: 2009-10-27
- Earliest claimed: 2002-05-24
- Assignees: Universidad Autonoma De Madrid
- Inventors: Jose Manuel Cuezva Marcos
- Web page: https://patentgrep.com/patent/US7608403B2

## CPC

- C12N 9/14: Hydrolases (3)
- G01N 33/57515: of the breast
- G01N 33/5752: of the lungs
- G01N 33/57525: of the liver or pancreas
- G01N 33/5753: of the stomach or small intestine
- G01N 33/57535: of the large intestine, e.g. colon, rectum or anus
- G01N 33/5758: {involving compounds serving as markers for tumours, cancers or neoplasias, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides or metabolites}
- G01N 2333/914: Hydrolases (3)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Described is a method for cancer detection, progression analysis and malign tumor prognosis based on the study of metabolic markers of the cell. The method consists in using the study of the protein expression of the bioenergetic function of the mitochondrion, such as the beta-catalytic subunit of the H-ATP synthase, in relation to structural proteins and mitochondrial respiration, such as hsp 60 and cytochrome oxidase subunits I and IV respectively, and said mitochondrial bioenergetic index, (ratio of beta-ATPasa/hsp60; beta-ATPasa/COX I; beta-ATPasa/COX IV) which relates to the cellular protein expression of the glycolytic pathway, such as glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase M, in order to generate the bioenergetic index of the cell.

## Claims

### Claim 1

A method for cancer detection, cancer progression analysis and malign tumor progression in cancer cells, comprising the steps of:
detecting the expression level of the polypeptide of subunit β of H(+)-ATP synthase of the cancer cells;
calculating a ration of the expression of β-F1-ATPase to Hsp60 of the cancer cells; and
comparing the ration with a ratio of β-F1-ATPase to Hsp60 of one of a) control non-cancerous cells of a same cellular type as the cancer cells and b) control cancer cells of a same cellular type as the cancer cells,
wherein, if the ratio of the cancer cells is lower than the ratio of the control non-cancerous cells, a presence of cancer is indicated, and
wherein, if the ratio of the cancer cells is lower than the ratio of the control cancer cells, that the cancer cells are further progressed cancer cells is implied and a subject from which the cancer cells were obtained has a worse prognosis than a subject from which the control cancer cells were obtained is implied.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the detecting step includes a use of immunological techniques.

### Claim 3 (depends on 2)

The method according to claim 2, wherein the immunological techniques are immunological techniques selected from a group consisting of: a Western blot, an immunohistochemical technique, and an immunocytochemical technique.