# US 7,608,283 B2 · Coral purification method and coral thus obtained

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 7,608,283 B2
- Document ID: US7608283B2
- Status: Granted
- Kind: B2
- Application number: 10/581,273
- Filed: 2004-12-06
- Published: 2007-05-03
- Granted: 2009-10-27
- Earliest claimed: 2003-12-05
- Assignees: Biocoral Inc.
- Inventors: Yann Lepetitcorps; Jean-Christophe Fricain; Vincent Souillac; Alain Largeteau; Roland Schmitthaeusler
- Web page: https://patentgrep.com/patent/US7608283B2

## CPC

- A61L 27/3691: {characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation}
- A61L 27/12: Phosphorus-containing materials, e.g. apatite
- A61L 27/3604: {characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel}
- A61L 27/365: {Bones}
- A61L 27/3687: {characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents}
- A61L 2430/02: for reconstruction of bones; weight-bearing implants
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)

## Abstract

The present invention provides a method of extracting organic substances present in coral, which consists in treating the coral with a fluid or a mixture of fluids in the supercritical state without modifying the crystalline structure of said coral, at a temperature of less than 270° C. and at a pressure which is much higher than the critical pressure of said fluid or mixture of fluids, for example of the order of at least 3 times, and preferably at least 5 times said critical pressure. It also provides coral obtained by said method and bone substitutes fabricated from said coral.

## Claims

### Claim 1

A method of extracting organic substances present in coral, comprising treating the coral with a fluid or said fluid in a mixture of fluids in a supercritical state without modifying the crystalline structure of said coral, at a temperature of less than 270° C., and at a pressure which is higher than the critical pressure of said fluid or mixture of fluids.

### Claim 2 (depends on 1)

A method according to claim 1, in which said fluid is selected from the group consisting of ethanol and acetone.

### Claim 3 (depends on 1)

A method according to claim 1, in which said fluid is ethanol.

### Claim 4 (depends on 3)

A method according to claim 3, in which the coral treatment pressure is in the range 300 MPa to 450 Mpa.

### Claim 5 (depends on 4)

A method according to claim 4, in which the coral treatment temperature is in the range 240° C. to 260° C., and the coral is treated for a period in the range 15 min to 240 min.

### Claim 6 (depends on 1)

A method according to claim 1, in which the mixture of fluids is an ethanol and carbon dioxide mixture, and the coral treatment pressure is in the range 30 MPa to 50 MPa.

### Claim 7 (depends on 6)

A method according to claim 6, in which the coral treatment temperature is of the range of 80° C. to 100° C.

### Claim 8 (depends on 1)

A method according to claim 1, in which said mixture of fluids is selected from the group consisting of ethanol and carbon dioxide mixtures, and acetone and carbon dioxide mixtures, the critical temperature of said mixture being less than 270° C.

### Claim 9 (depends on 3)

A method according to claim 3, in which the coral treatment pressure is in the range 350 MPa to 400 MPa.

### Claim 10

A method of extracting organic substances present in coral, comprising treating the coral with a fluid or said fluid in a mixture of fluids in a supercritical state without modifying the crystalline structure of said coral, at a temperature of less than 270° C. and at a pressure which is at least 3 times higher than the critical pressure of said fluid or mixture of fluids.