# US 7,608,266 B2 · Yersinia species compositions > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 7,608,266 B2 - Document ID: US7608266B2 - Status: Granted - Kind: B2 - Application number: 11/866,474 - Filed: 2007-10-03 - Published: 2008-01-31 - Granted: 2009-10-27 - Earliest claimed: 2003-01-31 - Assignees: University of North Dakota - Inventors: Matthew L. Nilles; Jyl S. Matson - Web page: https://patentgrep.com/patent/US7608266B2 ## CPC - C07K 16/1228: Enterobacterales (O), e.g. Citrobacter (G), Serratia (G), Proteus (G), Providencia (G), Morganella (G) or Yersinia (G) - A61K 39/025: {Enterobacteriales, e.g. Enterobacter} - A61P 31/04: Antibacterial agents - A61K 2039/505: {comprising antibodies} - Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE ## Abstract A method of protecting an animal from infections with pathogens originating from Yersinia comprising administering an isolated or recombinant YscF protein to the animal is disclosed. The isolated or recombinant YscF protein may be administered as a vaccine. An isolated or recombinant YscF protein capable of conferring protection to an animal against a pathogen of a Yersinia origin is further disclosed. Nucleic acid molecules encoding the isolated or recombinant YscF protein are also disclosed. In other embodiments, antibodies generated against the isolated or recombinant YscF protein capable of conferring protection to an animal against a pathogen or a Yersinia origin and uses of the antibodies are described. ## Claims ### Claim 1 An immunogenic composition comprising: a means for providing protection to an animal against a pathogen of Yersinia origin, the means for providing protection comprising an isolated or recombinant YscF protein comprising SEQ ID NO: 19; and a pharmaceutically suitable excipient, wherein the protection may be assayed in a mouse challenge model. ### Claim 2 (depends on 1) The immunogenic composition of claim 1, further comprising LcrV, the F1 antigen, YopD, an attenuated Yersinia bacterium, a recombinant carrier bacterium including a nucleic acid encoding a YscF protein, an inactive or killed Yersinia bacterium or combinations thereof. ### Claim 3 (depends on 1) The immunogenic composition of claim 1, further comprising an adjuvant. ### Claim 4 (depends on 1) The immunogenic composition of claim 1, further comprising PrgI, MxiH, EscF or combinations thereof. ### Claim 5 (depends on 1) The immunogenic composition of claim 1, wherein the pathogen is Yersinia pestis. ### Claim 6 (depends on 1) The immunogenic composition of claim 1, wherein the means for providing protection to an animal against a pathogen of Yersinia origin is a His-tagged YscF protein. ### Claim 7 An immunogenic composition for providing protection to an animal against a pathogen of Yersinia origin comprising: a recombinant YscF protein comprising SEQ ID NO: 19; and a pharmaceutically suitable excipient, wherein the protection may be assayed in a mouse challenge model. ### Claim 8 (depends on 7) The immunogenic composition of claim 7, further comprising LcrV, the F1 antigen, YopD, an attenuated Yersinia bacterium, a recombinant carrier bacterium including a nucleic acid encoding a YscF protein, an inactive or killed Yersinia bacterium or combinations thereof. ### Claim 9 (depends on 7) The immunogenic composition of claim 7, further comprising an adjuvant. ### Claim 10 (depends on 7) The immunogenic composition of claim 7, further comprising PrgI, MxiH, EscF or mixtures thereof. ### Claim 11 (depends on 7) The immunogenic composition of claim 7, wherein the recombinant YscF comprises His-tagged YscF. ### Claim 12 A composition produced by a process, the process comprising: providing a host cell with an expression vector comprising a nucleotide sequence encoding a YscF protein capable of providing protection to an animal against a pathogen of Yersinia origin, the nucleotide sequence encoding a YscF protein comprising SEQ ID NO: 19; expressing the nucleotide sequence in the host cell to produce the YscF protein; collecting the YscF protein; and mixing the collected YscF protein with a suitable excipient. ### Claim 13 (depends on 12) The composition produced by the process of claim 12, further comprising mixing LcrV, the Fl antigen, YopD or combinations thereof with the suitable excipient. ### Claim 14 (depends on 12) The composition produced by the process of claim 12, further comprising mixing an adjuvant with the suitable excipient. ### Claim 15 (depends on 12) The composition produced by the process of claim 12, further comprising mixing PrgI, MxiH, EscF or combinations thereof with the suitable excipient. ### Claim 16 A composition comprising: an isolated or recombinant YscF protein capable of providing protection to an animal against a pathogen of Yersinia origin, the isolated or recombinant YscF protein comprising SEQ ID NO: 19, wherein the protection may be assayed in a mouse challenge model; and a pharmaceutically acceptable excipient. ### Claim 17 (depends on 1) The immunogenic composition of claim 1, wherein the pathogen is selected from the group consisting of Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica. ### Claim 18 A kit for detecting an antibody capable of binding a YscF protein, the kit comprising: an isolated or recombinant YscF protein comprising SEQ ID NO: 19; means for detecting binding between the isolated or recombinant YscF protein and the antibody capable of binding the YscF protein; and a suitable liquid vehicle.