# US 7,608,258 B2 · Method for treatment of tendinosis using platelet rich plasma

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- Number: US 7,608,258 B2
- Document ID: US7608258B2
- Status: Granted
- Kind: B2
- Application number: 11/085,987
- Filed: 2005-03-22
- Published: 2005-08-25
- Granted: 2009-10-27
- Earliest claimed: 2002-04-13
- Inventors: Allan Mishra
- Web page: https://patentgrep.com/patent/US7608258B2

## CPC

- A61K 35/16: Blood plasma; Blood serum (umbilical cord blood A61K35/51)
- A61K 38/1841: {Transforming growth factor [TGF]}
- A61K 38/1858: {Platelet-derived growth factor [PDGF]}
- A61K 38/30: Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2 {(insulin-like growth factor binding protein A61K38/1754)}
- A61K 38/363: {Fibrinogen}
- A61K 38/39: Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
- A61K 38/4833: {Thrombin (3.4.21.5)}
- A61P 9/00: Drugs for disorders of the cardiovascular system
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

Methods and kits are disclosed to promote vascularization of tissue in mammals using an autologous platelet-rich plasma composition. The kit includes components for the preparation of platelet-rich plasma from the patient's blood and components for administration to an area in need of vascularization. The methods and kits are useful for the treatment of tendinosis, and in particular, lateral epicondylitis and plantar fasciitis.

## Claims

### Claim 1

A method of vascularizing an area of tissue injury in a mammal comprising:
drawing autologous blood from the mammal;
adding an anticoagulant to the autologous blood;
fractioning the autologous blood in a disposable separating tube using a centrifugation device to isolate a platelet-rich plasma composition;
titrating the platelet-rich plasma composition to obtain a pH of about 6.5 to 8.0 creating a buffered platelet-rich plasma; and
administering the buffered platelet-rich plasma to the area of tissue injury of the mammal, whereby platelet growth factors are released from the buffered platelet-rich plasma,
wherein no exogenous activator is administered to the mammal or added to the platelet-rich plasma composition prior to its introduction to tissue with a compromised blood supply, and wherein the tissue with has a compromised blood supply comprises a tendinosis.

### Claim 2 (depends on 1)

The method of claim 1, further comprising introducing a composition comprising a local anesthetic agent into the tissue before administering the buffered platelet-rich plasma composition.

### Claim 3 (depends on 1)

The method of claim 1, wherein the mammal is selected from the group consisting of horses, cows, sheep, pigs, dogs and cats.

### Claim 4 (depends on 1)

The method of claim 1, wherein the mammal is a human.

### Claim 5 (depends on 1)

The method of claim 1, wherein the tendinosis is tennis elbow.

### Claim 6 (depends on 1)

The method of claim 1, wherein the tendinosis is plantar fasciitis.

### Claim 7 (depends on 6)

The method of claim 6, further comprising identifying an area to be treated using ultrasound.

### Claim 8 (depends on 7)

The method of claim 7, wherein the buffered platelet-rich plasma composition is administered to a medial and/or central band of the identified fascia.

### Claim 9 (depends on 1)

The method of claim 1, wherein the tendinosis is rotator cuff injury.

### Claim 10 (depends on 1)

The method of claim 1, further comprising performing physical therapy to promote vascularization of the tendinosis.

### Claim 11

A method of treating a tendinosis in a mammal consisting of:
determining a site of tendinosis in the mammal;
administering a composition consisting of an anesthetic selected from the group consisting of anbesol, benzocaine, lidocaine, procaine, and bupivicaine, to the tendinosis site; and
administering a platelet-rich plasma composition without activator to the tendinosis site in the mammal.

### Claim 12 (depends on 11)

The method of claim 11, further comprising titrating the platelet-rich plasma composition to obtain a pH of about 6.5 to 8.0.