# US 7,608,189 B2 · Device for removing bacterial lipopolysaccharides and/or lipoteichoic acids from protein-containing fluids and its use for the treatment of sepsis

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- Number: US 7,608,189 B2
- Document ID: US7608189B2
- Status: Granted
- Kind: B2
- Application number: 10/860,786
- Filed: 2004-06-03
- Published: 2005-01-13
- Granted: 2009-10-27
- Earliest claimed: 2004-06-03
- Assignees: B. Braun Medizintechnologie GmbH
- Inventors: Dietrich Seidel; Karl-Siegfried Boos
- Web page: https://patentgrep.com/patent/US7608189B2

## CPC

- B01D 61/00: Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor (separation of gases or vapours by diffusion B01D53/22)
- A61M 1/3486: {Biological, chemical treatment, e.g. chemical precipitation; treatment by absorbents}
- A61M 1/3679: {by absorption (A61M1/3675 takes precedence)}
- B01D 67/00931: {by introduction of specific groups after membrane formation, e.g. by grafting}
- B01D 69/08: Hollow fibre membranes (manufacture of hollow fibres D01D5/24, D01F1/08)
- B01D 71/56: Polyamides, e.g. polyester-amides
- B01D 71/82: characterised by the presence of specified groups, e.g. introduced by chemical after-treatment
- A61M 2202/203: Bacteria
- B01D 2323/38: Graft polymerization
- B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28)
- A61M: DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY (introducing media into or onto the bodies of animals A61D7/00; means for inserting tampons A61F13/26; devices for administering food or medicines orally A61J; containers for collecting, storing or administering blood or medical fluids A61J1/05); DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY (surgery A61B; chemical aspects of surgical articles A61L); DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR {(Electrotherapy, e.g. producing anaesthesia by the use of alternating or intermittent currents A61N1/36021)}

## Abstract

A device for removing bacterial lipopolysaccharides and lipoteichoic acids from blood or plasma in an extracorporeal perfusion system contains, in a housing that can be incorporated into the perfusion system, a hollow fiber material that is suitable for the selective removal of bacterial lipopolysaccharides and lipoteichoic acids, the device being arranged in such a way that the blood or plasma entering through a first opening of the housing must pass through the hollow fiber material before it leaves through a second opening of the housing and is directed to the rest of the perfusion circuit.

## Claims

### Claim 1

A method of treating sepsis comprising treating a patient with sepsis by passing a body fluid of the patient through a device comprising a chemically modified hollow fiber material that is suitable for the collective removal of bacterial lipoteichoic acid and lipopolysacoharide such that the body fluid passes though said hollow fiber material and said hollow fiber material removes both bacterial lipoteichoic acid and lipopolysaccharide from the body fluid.

### Claim 2 (depends on 1)

The method of claim 1, wherein the hollow fiber materials used are selected from the group consisting of polyamides, polysulfones, polyethers, polyethylene, polypropylene, polyesters, derivatives and mixtures thereof.

### Claim 3 (depends on 1)

The method of claim 1, wherein the hollow fiber material is a polyamide.

### Claim 4 (depends on 1)

The method of claim 1, wherein the hollow fiber material is modified by graft polymerization.

### Claim 5 (depends on 1)

The method of claim 1, wherein tentacles, which comprise anion exchanger groups, are polymerized onto the hollow fiber material.

### Claim 6 (depends on 5)

The method of claim 5, wherein the anion exchanger groups comprise at least one of a synthetic, a semisynthetic, and a natural polycationic chain, said chain being in linear or branched form.

### Claim 7 (depends on 5)

The method of claim 5, wherein the anion exchanger groups comprise cationic or polycationic chains having tertiary or quaternary amines.

### Claim 8 (depends on 5)

The method of claim 5, wherein the anion exchanger groups are selected from the group consisting of di- or trialkylaminoalkyl, di- or trialkylaminoaryl, di- or triarylaminoalkyl, di- or triarylaminoaryl, di- or trialkylammoniumalkyl, di- or triarylammoniumalkyl, di- or triarylammoniumaryl, di- or trialkylammoniumaryl residues, polymers from amino acids that are positively charged or contain tertiary or quaternary amino groups, and polyethylene-imine.

### Claim 9 (depends on 1)

The method of claim 1, wherein the hollow fiber material comprises a polyamide modified with diethylaminoethyl groups.

### Claim 10 (depends on 1)

The method of claim 1 , wherein the device is arranged in the form of a filter module.

### Claim 11 (depends on 10)

The method of claim 10, wherein the filter module has a dead volume of <150 ml.

### Claim 12 (depends on 1)

The method of claim 1, wherein the device permits a flow rate of up to 200 ml/minute.

### Claim 13 (depends on 1)

The method of claim 1, wherein said device is designed for application in a plasma perfusion system.

### Claim 14 (depends on 1)

The method of claim 1, wherein said device is suitable for the treatment of diseases caused by at least one of gram-negative or gram-positive bacteria.

### Claim 15

A method of treating sepsis comprising treating a patient with sepsis by passing a body fluid of the patient through a device for removing both bacterial lipopolysaccharides lipoteichoic acids from a body fluid, said device comprising a housing that can be incorporated into the perfusion system, a chemically modified hollow fiber material that is suitable for the collective removal of both bacterial lipoteichoic acids and lipopolysaceharides in the housing, the device being arranged such that the blood or plasma entering through a first opening of the housing must pass through the hollow fiber material before it leaves through a second opening of the housing and is directed to the rest of the perfusion system, wherein said hollow fiber material comprises at least one polymer selected from the group consisting of polyamide, polysulfone, polyether, polyethylene, polypropylene, polyester, derivatives and mixtures thereof, wherein said hollow fiber material has tentacles comprising an anion exchange group polymerized thereon by graft polymerization, wherein the anion exchange group comprises at least one of the group consisting of a synthetic polycationic chain, a semisynthetic polycationic chain and a natural polycationic chain, wherein said synthetic, semisynthetic and natural polycationic chains are linear or branched and wherein said device has a dead volume <150 ml.

### Claim 16 (depends on 15)

The method of claim 15, wherein the device is used in an extracorporeal perfusion system.

### Claim 17 (depends on 15)

The method of claim 15, wherein the patient is afflicted with septic shock.

### Claim 18 (depends on 15)

The method of claim 15, wherein the body fluid is blood or plasma.

### Claim 19 (depends on 15)

The method of claim 15, wherein the device is a replaceable filter cartridge.

### Claim 20 (depends on 15)

The method of claim 15, wherein the hollow fiber material comprises nylon.

### Claim 21 (depends on 15)

The method as claimed in claim 15, wherein the anion exchange groups comprise a synthetic polycationic chain.

### Claim 22 (depends on 15)

The method as claimed in claim 15, wherein the anion exchange groups comprise a cationic chain or a polycationic chain including at least one of a tertiary amine or a quaternary amine.

### Claim 23 (depends on 15)

The method as claimed in claim 15, wherein the anion exchange groups are selected from the group consisting of di- or trialkylaminoalkyl, di- or trialkylaminoaryl, di- or triarylaminoalkyl, di- or triarylaminoaryl, di- or trialkylammoniumalkyl, di- or triarylammoniumalkyl, di- or triarylammoniumaryl or di- or trialkylammoniumaryl residues, polymers from amino acids that are positively charged or contain a tertiary or a quarternary amino group, and polyethylene-imine.

### Claim 24 (depends on 15)

The method as claimed in claim 15, wherein the hollow fiber material comprises a polyamide modified with diethylaminoethyl groups.

### Claim 25 (depends on 15)

The method as claimed in claim 15, wherein the hollow fiber material permits a flow rate of up to 200 ml/minute.

### Claim 26 (depends on 5)

The method of claim 5, wherein the anion exchanger groups are positively charged.

### Claim 27 (depends on 26)

The method of claim 26, wherein the anion exchanger groups are at least one of a tertiary amine and a quaternary amine.