Maize promoter active in silks, stalk nodes, roots and leaf sheaths
AssigneePioneer Hi-Bred International, Inc.
InventorsVirginia Crane, Douglas A. Rice, Jeanne Sandahl
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Abstract
The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a tissue-preferred maize promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
Claims12
A method for expressing a nucleotide sequence in a plant, said method comprising introducing into a plant a DNA construct, said DNA construct comprising a promoter and operably linked to said promoter a heterologous nucleotide sequence of interest, wherein said heterologous sequence is expressed in said plant, further wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5 or SEQ ID NO:
- 6;
- and
- b) a nucleotide sequence comprising the plant promoter sequence comprised in Patent Deposit No. NRRL B-30879.
A method for expressing a nucleotide sequence in a plant cell, said method comprising introducing into a plant cell a DNA construct comprising a promoter operably linked to a heterologous nucleotide sequence of interest, wherein said heterologous sequence is expressed in said plant, further wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5 or SEQ ID NO:
- 6;
- and
- b) a nucleotide sequence comprising the plant promoter sequence comprised in Patent Deposit No. NRRL B-30879.
Dependencies are inferred from the claim text.