Genetic insulator for preventing influence by another gene promoter
AssigneeUniversity of Kentucky Research Foundation
InventorsSusheng Gan, Mingtang Xie
Life of this patent
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Abstract
A 16 bp polynucleotide sequence of Arabidopsis thaliana is a genetic insulator that can effectively isolate a transgene from positional effects of neighboring gene activities in transgenic plant cells.
Claims4
A method for insulating the expression of a transgenic polypeptide from cis acting regulatory elements in the plant chromosome into which a polynucleotide coding for the expressed transgenic polypeptide has integrated, comprising:
- transfecting a plant cell with a polynucleotide construct comprising
- (a) a genetic insulator, comprising at least one copy of a polynucleotide having the sequence set forth in SEQ ID NO:9;
- (b) a transcription initiation region;
- and
- (c) a structural polynucleotide encoding the transgenic polypeptide;
- wherein the expression of the transgenic polypeptide from the integrated polynucleotide is insulated from cis acting regulatory elements in the plant chromosome into which the polynucleotide coding for the expressed polypeptide has integrated, and
- wherein, the transcription initiation region is heterologous to the genetic insulator comprising SEQ ID:
- 9.
A method for insulating the expression of a transgenic polypeptide from cis acting regulatory elements in the plant chromosome into which a polynucleotide coding for the expressed transgenic polypeptide has integrated, comprising:
- transfecting a plant cell with a polynucleotide construct comprising
- (a) a genetic insulator, comprising more than one copy of a polynucleotide having the sequence set forth in SEQ ID NO:9;
- (b) a transcription initiation region;
- and
- (c) a structural polynucleotide encoding the transgenic polypeptide;
- wherein the expression of the transgenic polypeptide from the integrated polynucleotide is insulated from cis acting regulatory elements in the plant chromosome into which the polynucleotide coding for the expressed polypeptide has integrated, and
- wherein, the transcription initiation region is heterologous to the genetic insulator comprising SEQ ID:
- 9.
Dependencies are inferred from the claim text.