Method of generating islet beta-cells from exocrine pancreatic cells
AssigneesVrije Universiteit Brussel, Opus, Inc.
InventorsLuc Bouwens, Luc Baeyens
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Abstract
The present invention discloses an in vitro method wherein mammalian beta-cell differentiation can be induced in dedifferentiated exocrine pancreatic cells in a medium comprising ligands of the EGF receptor and the GP130 receptor, such as EGF and LIF. Insulin secreting cells, obtainable by this method, provide a means for the treatment of diabetes by islet transplantation.
Claims12
An In vitro method of generating insulin producing beta cells from a population comprising dedifferentiated exocrine pancreatic cells of a first mammal, said method comprising the steps of:
- a) providing a population comprising dedifferentiated exocrine pancreatic cells in a culture medium,
- b) adding one or more ligands of the gp130 receptor of a second mammal and adding one or more ligands of the EGF receptor of a third mammal to said culture medium,
- c) incubating said dedifferentiated exocrine pancreatic cells in said culture medium comprising said one or more ligands of the gp130 receptor and said one or more ligands of the EGF receptor,
- wherein one of said one or more ligands of the EGF receptor is EGF.
A population of mammalian pancreatic cells comprising mammalian insulin producing beta cells obtainable by an in vitro method of generating insulin producing beta cells from a population comprising dedifferentiated exocrine pancreatic cells of a first mammal, said method comprising the steps of:
- a) providing a population comprising dedifferentiated exocrine pancreatic cells in a culture medium,
- b) adding one or more ligands of the gp130 receptor of a second mammal and adding one or more ligands of the EGF receptor of a third mammal to said culture medium,
- c) incubating said dedifferentiated exocrine pancreatic cells in said culture medium comprising said one or more ligands of the gp130 receptor and said one or more ligands of the EGF receptor,
- wherein one of said one or more ligands of the EGF receptor is EGF.
Dependencies are inferred from the claim text.