Methods of regulating differentiation in stem cells
AssigneeES Cell International PTE Ltd.
InventorsAlice Marie Pebay, Martin Frederick Pera
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Abstract
The present invention provides methods, media and compositions capable of modulating the differentiation of stem cells. Applicants have discovered that agonists of lysophospholipid receptors and ligands of class III tyrosine kinase receptors are useful in preventing the spontaneous differentiation of stem cells. The ligands and agonists may be used alone, or in combination where they have a synergistic effect. Also provided are cells produced using the methods and media, and methods of treating stem cell related diseases using the compositions described herein. Methods of identifying compounds useful in finding other agents useful in the modulation of stem cell differentiation are also disclosed.
Claims25
A serum free or substantially serum free medium useful for modulating spontaneous differentiation of a stem cell, comprising an agonist of the LPL receptor and a ligand of a class III tyrosine kinase receptor, wherein the agonist is selected from the group consisting of S1P, dihydro-S1P, LPA, PAF and SPC.
A serum-free or substantially serum-free medium useful for modulating spontaneous differentiation of a stem cell, comprising an agonist of a LPL receptor wherein said agonist is PAF.
Dependencies are inferred from the claim text.