Protein expression system
AssigneeNational Centre for Cell Science
InventorsSanjeev Galande, Prabhat K. Purbey, Peter C. Jayakumar, Milind S. Patole
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Abstract
The present invention relates to proteomics, especially downstream processing of proteins. The present invention also relates to novel expression cassettes having unique cleavage sites for efficient removal of purification tags. The invention further provides expression systems and a methods for purifying and isolating proteins.
Claims10
A novel expression cassette comprising an oligonucleotide encoding for a cleavage recognition site by a caspase, operably linked to a sequence encoding for a fusion protein, said fusion protein comprising a protein of interest having no internal cleavage site for caspase optionally fused to a purification tag, wherein the oligonucleotide is SEQ ID NO:13 or SEQ ID NO:14 encoding the caspase recognition site V-E-M-D (SEQ ID NO:
- 1) i.e. Valine-glutamic acid-methionine-aspartic acid (SEQ ID NO:1) or val-glu-met-asp (SEQ ID NO:
- 1) or V-E-I-D (SEQ ID NO:
- 2), and wherein the protein of interest is selected from the group consisting of SATB1, p53, PARP, Ikaros, cMyb, cMyc and HDAC1.
Dependencies are inferred from the claim text.