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AssigneeAjinomoto Co., Inc.
InventorsJoanna Yosifovna Katashkina, Maria Grigorievna Lunts, Vera Georgievna Doroshenko, Svetlana Aleksandrovna Fomina, Aleksandra Yurievna Skorokhodova, Lirina Valerievna Ivanovskaya, Sergei Vladimirovich Mashko
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A method is provided for obtaining an L-amino acid or nucleic acid-producing bacterium belonging to the genus Escherichia with an optimized level of expression of the gene which influences the distribution of carbon flow, such as the sucAB genes, comprising introducing into the chromosome of the bacterium a set of in vitro constructed DNA fragments which contain regulatory elements for gene expression instead of the native elements of the regulatory region of the gene, and selecting the colonies with increased L-amino acid productivity. Also, a method is provided for producing an L-amino acid, such as L-glutamic acid, L-proline, L-arginine, L-glutamine, L-leucine, using the bacterium with an optimized level of expression of the sucAB gene.
An L-amino acid-producing E. coil comprising an optimized level of expression of the sucAB gene, and wherein said E. coli has an ability to produce an L-amino acid in greater amounts than a parental strain, and wherein the −35 region of a promoter for the sucAB gene on a chromosome in said E. coli comprises the sequence shown in SEQ ID NO:
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